Gentamicin Has No Significant Adverse Effect on Porcine Embryo Development In Vitro

IF 2.7 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Reproduction and Development Pub Date : 2024-12-29 DOI:10.1002/mrd.70008

Rylie S. Noland, Bethany K. Redel, Marissa G. LaMartina, Paula R. Chen, Randall S. Prather

{"title":"Gentamicin Has No Significant Adverse Effect on Porcine Embryo Development In Vitro","authors":"Rylie S. Noland, Bethany K. Redel, Marissa G. LaMartina, Paula R. Chen, Randall S. Prather","doi":"10.1002/mrd.70008","DOIUrl":null,"url":null,"abstract":"<p>The composition of the culture medium affects the viability and developmental competency of porcine embryos produced in vitro. Previous transcriptional profiling has identified areas of improvement in the formulation of these media. Xenobiotic metabolism-related genes were upregulated in in vitro-cultured porcine embryos compared to their in vivo-derived counterparts, and the antibiotic gentamicin is a component of porcine embryo culture media. While effective against a broad spectrum of bacteria, gentamicin has been observed to be toxic to rat embryos and may induce changes in gene expression in cell culture. The objective of this study was to determine if gentamicin has an adverse effect on the development of porcine embryos. After in vitro fertilization, presumptive zygotes were placed in either MU4 medium containing gentamicin or MU4 medium without gentamicin. No difference was detected in blastocyst development, total cell number, apoptotic index, or expression of 3 selected genes between embryos cultured with or without gentamicin (<i>p</i> > 0.05). Therefore, porcine embryos are able to tolerate the presence of 10 μg/mL gentamicin without significant impacts on blastocyst development rate, total cell number, or apoptosis.</p>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"91 12","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.70008","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Reproduction and Development","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mrd.70008","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The composition of the culture medium affects the viability and developmental competency of porcine embryos produced in vitro. Previous transcriptional profiling has identified areas of improvement in the formulation of these media. Xenobiotic metabolism-related genes were upregulated in in vitro-cultured porcine embryos compared to their in vivo-derived counterparts, and the antibiotic gentamicin is a component of porcine embryo culture media. While effective against a broad spectrum of bacteria, gentamicin has been observed to be toxic to rat embryos and may induce changes in gene expression in cell culture. The objective of this study was to determine if gentamicin has an adverse effect on the development of porcine embryos. After in vitro fertilization, presumptive zygotes were placed in either MU4 medium containing gentamicin or MU4 medium without gentamicin. No difference was detected in blastocyst development, total cell number, apoptotic index, or expression of 3 selected genes between embryos cultured with or without gentamicin (p > 0.05). Therefore, porcine embryos are able to tolerate the presence of 10 μg/mL gentamicin without significant impacts on blastocyst development rate, total cell number, or apoptosis.

Abstract Image

查看原文本刊更多论文

庆大霉素对猪胚胎体外发育无明显不良影响

培养基的组成影响体外培养的猪胚胎的生存能力和发育能力。以前的转录分析已经确定了这些介质的配制方面有待改进的领域。体外培养的猪胚胎与体内培养的猪胚胎相比，外源代谢相关基因表达上调，抗生素庆大霉素是猪胚胎培养基的一种成分。虽然对广泛的细菌有效，但庆大霉素已被观察到对大鼠胚胎有毒，并可能诱导细胞培养中基因表达的变化。本研究的目的是确定庆大霉素是否对猪胚胎的发育有不利影响。体外受精后，假定受精卵被放置在含有庆大霉素的MU4培养基或不含庆大霉素的MU4培养基中。加庆大霉素与不加庆大霉素培养的胚胎囊胚发育、细胞总数、凋亡指数及3个选定基因的表达均无差异（p > 0.05）。因此，猪胚胎能够耐受10 μg/mL庆大霉素的存在，而不会对囊胚发育率、细胞总数或凋亡产生显著影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Molecular Reproduction and Development 生物-发育生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Molecular Reproduction and Development takes an integrated, systems-biology approach to understand the dynamic continuum of cellular, reproductive, and developmental processes. This journal fosters dialogue among diverse disciplines through primary research communications and educational forums, with the philosophy that fundamental findings within the life sciences result from a convergence of disciplines. Increasingly, readers of the Journal need to be informed of diverse, yet integrated, topics impinging on their areas of interest. This requires an expansion in thinking towards non-traditional, interdisciplinary experimental design and data analysis.