Association between the nutritional and fermentation profiles with protein-related molecular structure using fourier transformed infrared spectroscopy of snowbird faba bean seeds. Effects of steam pressure toasting duration

IF 2.5 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Feed Science and Technology Pub Date : 2025-02-01 DOI:10.1016/j.anifeedsci.2025.116217

María E. Rodríguez Espinosa, Peiqiang Yu

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Abstract

Heat processing alters the physicochemical structure of feed and food, modifying the degradation and the total nutrient supply. The objective of this study was to determine the extent of molecular structure modifications related to steam pressure toasting (SPT) time (0–120 min) in Faba bean seeds (FBS, Vicia faba cv. Snowbird). Whole FBS (n = 3 years) were autoclaved at 121°C and analyzed with Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy in the mid-infrared (mid-IR) region. The amide to total carbohydrates (TC) area ratio decreased quadratically from 0.77 to 0.67 absorbance units (AU; P < 0.01), as well as the structural carbohydrates (STC) to TC area ratio (P < 0.01). Conversely, the cellulosic compounds (CEC) to STC area ratio increased quadratically from raw to 120 min of SPT (P = 0.01). The peak absorbance for amide I, II, β-sheet, and α-helix decreased quadratically with increasing SPT time (P < 0.01). Based on the multivariate analysis, T30, T60, and T90 did not share similar molecular spectra in the amide I area at ca. 1715–1584 cm⁻¹ (PC-1 =92 %). The rumen undegraded crude protein (BCP_DVE, g/kg DM) had an inverse relationship with α-helix (r = -0.66; P = 0.01), β-sheet (r = -0.56; P = 0.03), and amide and amide I areas (r = -0.61; P = 0.02). Similarly, the intestinal digestible crude protein (IADP, g/kg CP), the total metabolizable protein (g/kg DM), and the feed milk value (FMV, kg milk/kg DM feed) presented a negative correlation with all the protein-related molecular profiles (P < 0.05). The analysis of FTIR data allowed us to detect differences between the experimental treatments. The AU in areas and heights of amide I and II, α-helix and β-sheet heights, and the area ratio of amide to TC were lower with increasing SPT time. Moreover, a positive correlation between the EDCP and all the spectral profiles was observed.

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利用傅立叶变换红外光谱分析雪鸟蚕豆种子的营养和发酵特征与蛋白质相关分子结构的关系。蒸汽压力对烘烤时间的影响

热处理改变了饲料和食品的物理化学结构，改变了降解和总营养供应。本研究的目的是确定与蒸汽压力烘烤（SPT）时间（0-120 min）有关的蚕豆种子（FBS, Vicia Faba cv）分子结构修饰的程度。雪鸟)。整个FBS （n = 3年）在121°C下蒸压，用衰减全反射傅立叶变换红外（ATR-FTIR）光谱在中红外（中红外）区域进行分析。酰胺与总碳水化合物（TC）的面积比从0.77降至0.67；P <； 0.01)，以及结构碳水化合物（STC）与TC的面积比（P <； 0.01）。相反，纤维素化合物（CEC）与STC的面积比从原始SPT到120 min呈二次增长（P = 0.01）。酰胺I、II、β-sheet和α-helix的吸光度峰值随着SPT时间的增加呈二次曲线下降（P <； 0.01）。基于多变量分析，T30、T60和T90在约1715-1584 cm−1 （PC-1 =92 %）的酰胺I区没有相似的分子光谱。瘤胃未降解粗蛋白质（BCPDVE, g/kg DM）与α-螺旋呈反比关系(r = -0.66；P = 0.01),β-sheet (r = -0.56；P = 0.03)，酰胺和酰胺I区域(r = -0.61； = 0.02页)。肠道可消化粗蛋白质（IADP, g/kg CP）、总代谢蛋白质（g/kg DM）、料乳值（FMV， kg乳/kg DM饲料）与所有蛋白质相关分子谱均呈负相关（P <； 0.05）。FTIR数据的分析使我们能够发现实验处理之间的差异。随着SPT时间的延长，酰胺I和酰胺II的面积和高度、α-螺旋和β-片高度以及酰胺与TC的面积比均降低。此外，EDCP与所有谱线均呈正相关。

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来源期刊

Animal Feed Science and Technology 农林科学-奶制品与动物科学

CiteScore

6.00

自引率

6.20%

发文量

266

审稿时长

3 months

期刊介绍： Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.