Phosphorylated chitosan from the internal bone of Sepia kobiensis (Hoyle, 1885) and their inhibition against oral pathogens

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2025-02-04 DOI:10.1016/j.carres.2025.109413

Preetha Srinivasan , Annathai Pitchai , Pasiyappazham Ramasamy

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Abstract

Phosphorylated chitosan, derived from the internal bone of Sepia kobiensis (Hoyle, 1885), has been investigated for its antimicrobial properties against oral pathogens. Chitosan's solubility in water and its biological effectiveness were enhanced through the alteration of its composition with orthophosphoric acid and dimethylformamide (DMF). Structural analysis for both chitosan and phosphorylated chitosan was performed using FTIR spectroscopy, field emission scanning electron microscopy (FESEM), and X-ray diffraction (XRD). The antimicrobial efficacy of chitosan and phosphorylated chitosan was assessed against clinically isolated human pathogens, including Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa, and Candida tropicalis. The highest inhibition zone, with a diameter of 23 ± 1.33 mm, was observed against P. aeruginosa for chitosan, while phosphorylated chitosan recorded a 25 ± 2.05 mm inhibition zone against E. coli. The findings of this study indicate that phosphorylated chitosan, with its potent antimicrobial properties, could serve as a possible antimicrobial drug for oral pathogens, sourced from a natural and sustainable origin.

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".