A nano drug delivery system loading drugs and chlorin e6 separately to achieve photodynamic-chemo combination therapy.

Nanomedicine (London, England) Pub Date : 2025-03-01 Epub Date: 2025-02-04 DOI:10.1080/17435889.2025.2460960

Guohao Yin, Hongli Zhao, Minbo Lan

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Abstract

Aim: To develop a new drug delivery system (DDS) that can load chemotherapy agents and photosensitizer chlorin e6 (Ce6) onto the pores and surfaces of mesoporous silica nanoparticle (MSN) separately.

Methods: Doxorubicin (DOX) was loaded into the pores of MSNs. Then, polyethyleneimine (PEI) was used to coat the surface of MSN to protect DOX, and then manganese dioxide (MnO₂) nanoparticles were loaded through adding potassium permanganate (KMnO₄) to bind with Ce6. Finally, polydopamine (PDA) was coated and coupled with hyaluronic acid (HA).

Results: The synthesized versatile nanoparticle was pH-sensitive and exhibited positive photodynamic therapy (PDT) performance. Besides, it could be observed that the nanoparticles were efficiently taken up by tumor cells through confocal laser scanning microscopy (CLSM) and flow cytometry. Additionally, in vitro experiments suggested that the nanoparticles had pleasing toxicity to various tumor cells and equally positive therapeutic effect when curcumin replaced DOX.

Conclusion: Our work suggests that the nanoparticles designed by our strategy have satisfactory combination therapy performance and can enable more chemotherapy drugs to be used in photodynamic-chemo combination therapy.

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分别装载药物和氯素 e6 的纳米给药系统，实现光动力-化疗联合疗法。

目的：开发一种新型给药系统（DDS），该系统可将化疗药物和光敏剂氯素e6（Ce6）分别负载到介孔二氧化硅纳米粒子（MSN）的孔隙和表面：方法：将多柔比星（Doxorubicin，DOX）载入介孔二氧化硅纳米粒子（MSN）的孔隙中。然后用聚乙烯亚胺（PEI）包覆 MSN 表面以保护 DOX，再通过添加高锰酸钾（KMnO4）使二氧化锰（MnO2）纳米粒子与 Ce6 结合。最后，聚多巴胺（PDA）被包覆并与透明质酸（HA）耦合：结果：合成的多功能纳米粒子对 pH 值敏感，具有良好的光动力疗法（PDT）性能。此外，通过激光共聚焦扫描显微镜（CLSM）和流式细胞术可以观察到纳米颗粒被肿瘤细胞有效吸收。此外，体外实验表明，纳米颗粒对各种肿瘤细胞的毒性不明显，当姜黄素替代 DOX 时，治疗效果同样良好：我们的工作表明，我们的策略所设计的纳米颗粒具有令人满意的联合治疗性能，可以使更多的化疗药物用于光动力-化疗联合治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nanomedicine (London, England)

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