Evaluation of a fluorophore for marking navel orangeworm (Lepidoptera: Pyralidae).

IF 2.1 3区 农林科学 Q1 ENTOMOLOGY
Tzu-Chin Jean Liu, Charles S Burks, Houston Wilson
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引用次数: 0

Abstract

Navel orangeworm, Amyelois transitella (Walker), is a key pest in California's almonds, pistachios, and walnuts. This insect's strong dispersal capacity can potentially undermine the efficacy of localized management efforts. The timing and extent of A. transitella movement between orchards remain unclear, and more studies are needed to better characterize its landscape ecology. Mark-release-recapture studies offer a potential solution but require a reliable insect marker that is durable, easily identifiable and has minimal impacts on A. transitella longevity and flight ability. To address this, we evaluated 4 colors (red, blue, green, and yellow) of a fluorophore marker (SmartWater) for adult A. transitella. We conducted laboratory assays to assess moth flight ability and mortality, as well as marker persistence over time using both quantitative (plate reader) and qualitative (visual observation) fluorophore detection methods. Results demonstrated that none of the 4 colors negatively affected A. transitella flight ability or mortality. Green and yellow markers were persistent and readily identified by both detection methods, unlike blue and red markers. Although marker degradation was observed over time with the quantitative method, a high percentage of moths (70.3%) retained green and yellow markers after 14 days. In contrast, these markers did not show significant degradation using the qualitative method, with over 94.2% of moths showing fluorescence 14 days postmarking. These findings highlight the strong potential of green and yellow markers for field studies with A. transitella. We discuss their use in future mark-release-recapture studies and compare the 2 fluorophore detection methods.

一种荧光标记脐橙虫(鳞翅目:蚜科)的评价。
脐橙虫,是加州杏仁、开心果和核桃的主要害虫。这种昆虫强大的传播能力可能会潜在地破坏局部管理努力的效果。越境小蠊在果园间迁移的时间和范围尚不清楚,需要更多的研究来更好地表征越境小蠊的景观生态。标记释放-再捕获研究提供了一种潜在的解决方案,但需要一种可靠的昆虫标记,这种标记持久、易于识别,并且对transitella的寿命和飞行能力影响最小。为了解决这个问题,我们对一种荧光标记物(SmartWater)的4种颜色(红、蓝、绿、黄)进行了评估。我们使用定量(平板阅读器)和定性(目视观察)荧光团检测方法进行了实验室分析,以评估飞蛾的飞行能力和死亡率,以及标记物随时间的持久性。结果表明,4种颜色均不影响飞蛾的飞行能力和死亡率。与蓝色和红色标记不同,绿色和黄色标记持久且易于被两种检测方法识别。虽然用定量方法观察到标记物随时间的降解,但在14天后,高比例的飞蛾(70.3%)保留了绿色和黄色标记物。相比之下,使用定性方法,这些标记没有显示出明显的降解,超过94.2%的飞蛾在标记14天后显示荧光。这些发现突出了绿色和黄色标记在transitella实地研究中的巨大潜力。我们讨论了它们在未来的标记释放-再捕获研究中的应用,并比较了两种荧光团检测方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Insect Science
Journal of Insect Science 生物-昆虫学
CiteScore
3.70
自引率
0.00%
发文量
80
审稿时长
7.5 months
期刊介绍: The Journal of Insect Science was founded with support from the University of Arizona library in 2001 by Dr. Henry Hagedorn, who served as editor-in-chief until his death in January 2014. The Entomological Society of America was very pleased to add the Journal of Insect Science to its publishing portfolio in 2014. The fully open access journal publishes papers in all aspects of the biology of insects and other arthropods from the molecular to the ecological, and their agricultural and medical impact.
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