Gut microbial profiles of patients with optic neuritis or myasthenia gravis.

IF 1.4 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Tao Wu, Hanqiu Jiang, Chen Lin, Jingting Peng, Xiuyun Kong, Jie Yu, Jiawei Wang, Shilei Cui
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引用次数: 0

Abstract

Objective: To characterize the gut microbial composition of patients with optic neuritis (ON) or myasthenia gravis (MG).

Methods: Stool samples were collected from 45 patients with ON, 13 patients with MG, and 20 healthy controls. Microbial genomic DNA was extracted, and the V3-V4 regions of bacterial 16S rRNA genes were amplified and sequenced. Bioinformatic analyses was performed to compare the alpha-diversity, beta-diversity, taxonomic assignments, and bacterial richness of the groups. Differences in the abundances of microbial taxa were identified using linear discriminant analysis effect size (LEfSe) and analysis of variance.

Results: Beta-diversity analysis showed distinct clustering of patient samples from the healthy controls. At the phylum and genus levels, Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria predominated, but their proportions varied between groups. LEfSe analysis identified microbial taxa that were associated with each group. The patients showed lower abundances of certain intestinal probiotics, including Bifidobacterium, Bacteroides, and Roseburia, than the controls. No significant differences were found between the disease subgroups. The Carnobacteriaceae family was significantly less abundant in the ON than in the MG group.

Conclusion: We have identified significant alterations in the gut microbiota of patients with ON or MG, and importantly, a notable reduction in intestinal probiotics.

视神经炎或重症肌无力患者的肠道微生物谱。
目的:了解视神经炎(ON)或重症肌无力(MG)患者的肠道微生物组成。方法:收集45例ON患者、13例MG患者和20例健康对照者的粪便标本。提取微生物基因组DNA,扩增细菌16S rRNA基因v3 ~ v4区并测序。生物信息学分析比较了这两个群体的α -多样性、β -多样性、分类分配和细菌丰富度。利用线性判别分析(linear discriminant analysis, LEfSe)和方差分析(analysis of variance)对微生物类群丰度差异进行了鉴定。结果:β -多样性分析显示患者样本与健康对照组有明显的聚类。在门和属水平上,放线菌门、拟杆菌门、厚壁菌门和变形菌门占主导地位,但它们的比例在不同类群之间有所不同。LEfSe分析确定了与每组相关的微生物类群。与对照组相比,患者肠道中某些益生菌的丰度较低,包括双歧杆菌、拟杆菌和玫瑰菌。在疾病亚组之间没有发现显著差异。与MG组相比,ON组的肉杆菌科数量明显减少。结论:我们已经确定了ON或MG患者肠道微生物群的显著改变,重要的是,肠道益生菌显著减少。
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来源期刊
CiteScore
3.20
自引率
0.00%
发文量
555
审稿时长
1 months
期刊介绍: _Journal of International Medical Research_ is a leading international journal for rapid publication of original medical, pre-clinical and clinical research, reviews, preliminary and pilot studies on a page charge basis. As a service to authors, every article accepted by peer review will be given a full technical edit to make papers as accessible and readable to the international medical community as rapidly as possible. Once the technical edit queries have been answered to the satisfaction of the journal, the paper will be published and made available freely to everyone under a creative commons licence. Symposium proceedings, summaries of presentations or collections of medical, pre-clinical or clinical data on a specific topic are welcome for publication as supplements. Print ISSN: 0300-0605
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