TMEM119-positive microglial cells in cerebrospinal fluid, a potential new marker for neuroinflammatory response after aneurysmal subarachnoid hemorrhage.

IF 4 4区 医学 Q2 CLINICAL NEUROLOGY
Journal of Neural Transmission Pub Date : 2025-05-01 Epub Date: 2025-02-04 DOI:10.1007/s00702-025-02886-3
Andrea Cattaneo, Julia Messinger, Kevin Lamllari, Helmut Heinsen, Michael K Schuhmann, Christoph Wipplinger, Vera Nickl, Mario Löhr, Ekkehard Kunze, Christian Stetter, Thomas Linsenmann, Michael Bohnert, Ralf-Ingo Ernestus, Johann Zwirner, Benjamin Ondruschka, Camelia-Maria Monoranu, Simone Bohnert
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引用次数: 0

Abstract

Aneurysmal subarachnoid hemorrhage (aSAH) is a debilitating condition with significant morbidity and mortality rates. Despite advancements in treatment, understanding the underlying pathophysiology, particularly the inflammatory response, remains crucial for improving patient outcomes. In this study, we investigated the presence of transmembrane protein 119 (TMEM119) of microglial cells in cerebrospinal fluid (CSF) as a potential marker for neuroinflammation following aSAH. CSF samples were collected from aSAH patients, pathological and healthy controls, processed, and analyzed using immunocytochemistry. TMEM119-positive microglial cells were consistently identified in the CSF of aSAH patients, exhibiting amoeboid morphology and intense staining. Importantly, microglial cells were detected as early as the first day post-bleeding, persisting throughout the acute phase in some cases. Analysis of consecutive samples revealed varying trends in microglial cell numbers, with a peak during the initial phase followed by a gradual decline. Our findings suggest that microglia may migrate into the CSF following aSAH, potentially serving as an early predictor of inflammatory-related CNS damage. This study underscores the importance of understanding neuroinflammatory processes in aSAH and opens avenues for further research on the role of microglia in CNS disorders by liquid biopsy.

Abstract Image

Abstract Image

脑脊液中 TMEM119 阳性的小胶质细胞--动脉瘤性蛛网膜下腔出血后神经炎症反应的潜在新标记。
动脉瘤性蛛网膜下腔出血(aSAH)是一种使人衰弱的疾病,发病率和死亡率都很高。尽管治疗取得了进步,但了解潜在的病理生理学,特别是炎症反应,仍然是改善患者预后的关键。在这项研究中,我们研究了脑脊液(CSF)中小胶质细胞跨膜蛋白119 (TMEM119)作为aSAH后神经炎症的潜在标志物的存在。收集aSAH患者、病理对照和健康对照的脑脊液样本,使用免疫细胞化学进行处理和分析。aSAH患者脑脊液中一致发现tmem119阳性的小胶质细胞,呈变形虫形态,染色强烈。重要的是,小胶质细胞早在出血后第一天就被检测到,在一些病例中,小胶质细胞持续存在于整个急性期。对连续样品的分析揭示了小胶质细胞数量的变化趋势,在初始阶段达到峰值,随后逐渐下降。我们的研究结果表明,小胶质细胞可能在aSAH后迁移到脑脊液中,可能作为炎症相关中枢神经系统损伤的早期预测因子。这项研究强调了了解aSAH中神经炎症过程的重要性,并为通过液体活检进一步研究小胶质细胞在中枢神经系统疾病中的作用开辟了道路。
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来源期刊
Journal of Neural Transmission
Journal of Neural Transmission 医学-临床神经学
CiteScore
7.20
自引率
3.00%
发文量
112
审稿时长
2 months
期刊介绍: The investigation of basic mechanisms involved in the pathogenesis of neurological and psychiatric disorders has undoubtedly deepened our knowledge of these types of disorders. The impact of basic neurosciences on the understanding of the pathophysiology of the brain will further increase due to important developments such as the emergence of more specific psychoactive compounds and new technologies. The Journal of Neural Transmission aims to establish an interface between basic sciences and clinical neurology and psychiatry. It intends to put a special emphasis on translational publications of the newest developments in the field from all disciplines of the neural sciences that relate to a better understanding and treatment of neurological and psychiatric disorders.
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