Effect of surface coatings on endothelialization and biofilm in PTFE vascular grafts.

Abstract

Polytetrafluoroethylene (PTFE) grafts are of great importance for vascular surgery and many methods have been developed to improve their biocompatibility. The most important of these methods is the coating of the inner surfaces of the grafts. In this study, the effects of surface coatings used in vascular grafts on endothelialization and bacterial biofilm formation were investigated. Three different PTFE graft types, heparin coated, carbon coated and uncoated, were compared. HUVEC cell culture was used for endothelialization experiments and Staphylococcus aureus strain was used for biofilm formation. Endothelialization was evaluated by inverted microscopy and scanning electron microscopy (SEM). Heparin-coated grafts showed more biofilm formation than other graft types (p < 0.01). Moderate biofilm formation was observed in carbon-coated grafts (p < 0.05). When evaluating endothelialization, heparin-coated grafts showed more cell adhesion in the first days, but lagged behind the other graft types in the following days. Carbon-coated grafts showed more endothelial cell proliferation in the long term. While biofilm formation was high in heparin-coated grafts, carbon-coated grafts provided better endothelialization. Our study showed that the coating of PTFE grafts significantly affects biocompatibility and infection risk.