Efficacy of nitrofuran derivatives against biofilms of Histoplasma capsulatum strains and their in vivo toxicity.

IF 2.5 4区 生物学 Q3 MICROBIOLOGY
Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-02-05 DOI:10.1080/17460913.2025.2457286
Carolina Orlando Vaso, Níura Madalena Bila, Rosângela Aparecida Moraes da Silva, Angélica Romão de Carvalho, Jennyfie Araújo Belizário, Fabiana Pandolfi, Daniela De Vita, Martina Bortolami, Maria José Soares Mendes-Giannini, Luigi Scipione, Roberto Di Santo, Roberta Costi, Caroline Barcelos Costa-Orlandi, Ana Marisa Fusco-Almeida
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引用次数: 0

Abstract

Aim: To evaluate the efficacy of two nitrofuran derivatives against biofilms formed by two strains of Histoplasma capsulatum and to study the toxicity of these compounds in alternative models: Caenorhabditis elegans, Galleria mellonella, and zebrafish.

Methods: The metabolic activity of biofilms was measured after treatment using the XTT reduction assay. Scanning electron microscopy (SEM) and confocal microscopy were used to observe damage to mature biofilms. Survival curves were generated for G. mellonella, while percentage survival was determined for C. elegans and zebrafish.

Results: The compounds showed efficacy against early and mature biofilms at concentrations equal to or up to two times higher than those required to eliminate planktonic fungal cells (3.90 to 31.25 μg/mL). Micrographs showed a reduction in metabolic activity, biofilm thickness, and extracellular matrix. In addition, the compounds showed little or no toxicity in alternative models, even at the highest concentrations tested.

Conclusion: These results are promising for the development of new therapeutic alternatives, especially for species, such as H. capsulatum, which are recognized as high-priority pathogens. Few studies have investigated resistance and antifungal treatment targeting biofilms of this species, making this work a relevant contribution to future approaches.

硝基呋喃衍生物对荚膜组织浆菌生物膜的抑制作用及其体内毒性。
目的:评价两种硝基呋喃衍生物对两种荚膜组织原体形成的生物膜的抑制作用,并研究其对秀丽隐杆线虫、黑蜡虫和斑马鱼的毒性。方法:采用XTT还原法测定生物膜处理后的代谢活性。利用扫描电镜和共聚焦显微镜观察成熟生物膜的损伤情况。绘制了黄蜡线虫的存活率曲线,测定了秀丽隐杆线虫和斑马鱼的存活率。结果:该化合物对早期和成熟生物膜的杀伤作用浓度(3.90 ~ 31.25 μg/mL)等于或高达清除浮游真菌细胞所需浓度的2倍。显微照片显示代谢活性、生物膜厚度和细胞外基质减少。此外,在其他模型中,即使在测试的最高浓度下,这些化合物也显示出很少或没有毒性。结论:这些结果前途发展的新的治疗选择,尤其是对物种,如h . capsulatum,公认为高优先级病原体。很少有研究针对该物种的生物膜进行耐药性和抗真菌治疗,使这项工作对未来的方法做出了相关贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Future microbiology
Future microbiology 生物-微生物学
CiteScore
4.90
自引率
3.20%
发文量
134
审稿时长
6-12 weeks
期刊介绍: Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.
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