Effects of pentoxifylline on mouse oocytes maturation and quality in vitro.

IF 2.1 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Iranian Journal of Basic Medical Sciences Pub Date : 2025-01-01 DOI:10.22038/ijbms.2024.77926.16856

Junjiao Wu, Jianbo Wu, Yanyan Xu

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引用次数: 0

Abstract

Objectives: To investigate the impact of Pentoxifylline (PTX) on the in vitro maturation (IVM) of mouse oocytes and its effect on oocyte quality.

Materials and methods: This experimental study involved culturing mouse oocytes in an IVM medium with varying PTX concentrations (0-100 μM). Post-culture, oocytes were assessed for nuclear and cytoplasmic maturation and quality indicators, including germinal vesicle breakdown (GVBD), first polar body extrusion (PB1E), cortical granules (CGs) distribution, spindle structure, chromosome alignment, and intracellular reactive oxygen species (ROS) levels.

Results: Treatment with PTX at 10, 25, and 50 μM concentrations significantly enhanced the nuclear maturation rates of oocytes. The optimal concentration was found to be 10 μM, as it resulted in the most favorable cytoplasmic maturation, characterized by improved distribution of CGs, spindle structure, and chromosome alignment. Additionally, treatment with 10 μM PTX effectively reduced reactive oxygen species (ROS) levels.

Conclusion: PTX supplementation at specific concentrations enhances mouse oocyte maturation and quality, potentially by facilitating CG distribution, spindle integrity, and chromosome alignment and by reducing ROS production.

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己酮茶碱对小鼠卵母细胞体外成熟和质量的影响。

目的研究五氧化锡（Pentoxifylline，PTX）对小鼠卵母细胞体外成熟（IVM）的影响及其对卵母细胞质量的影响：本实验研究采用不同浓度的 PTX（0-100 μM）在 IVM 培养基中培养小鼠卵母细胞。培养后，对卵母细胞的核和胞质成熟度及质量指标进行评估，包括生殖泡破裂（GVBD）、第一极体挤出（PB1E）、皮质颗粒（CGs）分布、纺锤体结构、染色体排列和细胞内活性氧（ROS）水平：结果：10、25 和 50 μM 浓度的 PTX 能显著提高卵母细胞的核成熟率。最佳浓度为 10 μM，因为它能带来最有利的细胞质成熟，其特点是 CGs 分布、纺锤体结构和染色体排列得到改善。此外，10 μM PTX 能有效降低活性氧（ROS）水平：结论：补充特定浓度的 PTX 可促进 CG 分布、纺锤体完整性和染色体排列，并减少 ROS 的产生，从而提高小鼠卵母细胞的成熟度和质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY

CiteScore

4.00

自引率

4.50%

发文量

142

审稿时长

6-12 weeks

期刊介绍： The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.