COTI-2 suppresses the malignancy of bladder cancer by inducing apoptosis via the AMPK-mTOR signaling pathway.

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Yuancai Zheng, Keqi Wang, Chenyu Wu, Yuying Qin, Yihan Sun, Xinyu Lu, Yupeng Xu, Gonghui Li
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引用次数: 0

Abstract

Objectives: COTI-2, an innovative oral homocysteine, has shown promising antitumor results on multiple types of cancer. However, its effects in treating bladder cancer (BCa) and the underlying molecular mechanisms have not been elucidated. The present study aimed to explore the antitumor effects of COTI-2 on BCa and the potential mechanisms.

Materials and methods: BCa cell lines, including the 5637 and T24 cell lines, were treated with COTI-2 at concentrations of 0.5 and 1 μM, respectively. Cell Counting Kit (CCK)-8 assay, colony formation assay, apoptosis assay, and transwell migration and invasion assay were conducted to evaluate the antitumor effects of COTI-2 on BCa cells. Western blotting, H&E, immunohistochemical staining, and immunofluorescence analysis were performed to investigate the underlying mechanisms. Moreover, a xenograft model in nude mice using T24 cells was generated to determine the antitumor activities of COTI-2 in vivo.

Results: COTI-2 highly inhibited the proliferation of BCa cell lines, including 5637 and T24 cells, and induced their apoptosis. Moreover, it efficiently suppressed the migration and invasion of BCa cells. Additionally, the subcutaneous xenograft model in nude mice showed that COTI-2 treatment inhibited the tumor growth of BCa by inducing its apoptosis in vivo. We also found that COTI-2 promoted apoptosis in BCa cells, presumably through activating the AMPK/mTOR pathway.

Conclusion: Our data suggest that COTI-2 effectively reduces the malignancy of BCa, probably by inducing apoptosis via the AMPK/mTOR signaling pathway. These data highlight the potential of COTI-2 as a therapeutic agent for the treatment of BCa.

COTI-2通过AMPK-mTOR信号通路诱导细胞凋亡,从而抑制膀胱癌的恶性发展。
目的:COTI-2是一种创新的口服同型半胱氨酸,对多种类型的癌症显示出良好的抗肿瘤效果。然而,其治疗膀胱癌(BCa)的作用及其潜在的分子机制尚未阐明。本研究旨在探讨COTI-2对BCa的抗肿瘤作用及其可能的机制。材料和方法:分别用0.5 μM和1 μM浓度的COTI-2处理BCa细胞株5637和T24。采用细胞计数试剂盒(CCK)-8法、集落形成法、细胞凋亡法和跨井迁移侵袭法评价COTI-2对BCa细胞的抗肿瘤作用。Western blotting、H&E、免疫组织化学染色和免疫荧光分析探讨其潜在机制。此外,利用T24细胞建立裸鼠异种移植模型,测定COTI-2在体内的抗肿瘤活性。结果:COTI-2对BCa细胞株5637、T24细胞增殖有明显抑制作用,并诱导其凋亡。此外,它还能有效抑制BCa细胞的迁移和侵袭。此外,裸鼠皮下异种移植模型显示,COTI-2处理通过诱导BCa的体内凋亡来抑制BCa的肿瘤生长。我们还发现,COTI-2可能通过激活AMPK/mTOR通路,促进BCa细胞的凋亡。结论:我们的数据表明,COTI-2可能通过AMPK/mTOR信号通路诱导BCa凋亡,从而有效降低BCa的恶性程度。这些数据强调了COTI-2作为BCa治疗药物的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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