A Novel Strategy for Authentication and Quantification of Porcine and Water Buffalo Hide Gelatin in Processed Products Using Multiple Reaction Monitoring Mass Spectrometry Approaches

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2025-02-04 DOI:10.1002/elps.202400209

Bidyut Prava Mishra, Naveena B. Maheswarappa, B. Eswara Rao, Rituparna Banerjee, Prasad M. Govindaiah, Balaji B. Manohar, Prasana Kumar Rath

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引用次数: 0

Abstract

Pig skin and water buffalo hides were partially hydrolyzed to produce a heterogeneous mixture of polypeptides, known as gelatin, a commonly used food additive. Specific peptide markers were explored in the context of authentication and relative quantification of water buffalo hide gelatin (BHG) and porcine skin gelatin (PSG) via multiple reaction monitoring-mass spectrometry (MRM-MS). Tryptic gelatin peptides were separated and analyzed with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and five unique peptide biomarkers were selected for BHG and PSG. Gelatin extraction from BHG and PSG spiked chicken meat patties (CMP) followed by LC-MS/MS MRM-MS revealed two unique bovine gelatin peptides of m/z 781.336 and 852.718 and three unique porcine gelatin peptides of m/z 774.570, 971.776, and 727.436. The optimized MRM-MS protocol was efficient in detecting BHG/PSG from spiked CMP up to 0.5% (w/w). Twenty-five commercial food samples were screened, among which four samples showed the presence of BHG and six samples showed the presence of PSG. The proposed LC-MS/MS MRM strategy provides an efficient and sensitive authentication and traceability of gelatin-containing highly processed food, bakery, and confectionery products.

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.