Exosomes derived from follicular fluid promote proliferation and suppress apoptosis of yak (Bos grunniens) granulosa cells by enhancing antioxidant capacity in vitro

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2025-01-31 DOI:10.1016/j.anireprosci.2025.107790

Bangting Pan , Xupeng Li , Qinhui Yang , Yumian Xie , Zhuo Chen , Jun Yu , Honghong He , Yan Xiong , Wei Fu , Shi Yin , Daoliang Lan , Jian Li , Xianrong Xiong

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引用次数: 0

Abstract

Follicular fluid exosomes (FFEs) play a crucial role in follicular growth and maturation. As the fundamental component of the ovarian follicle, granulosa cells (GCs) are instrumental in the development of follicles and oocytes. This study explored the effects of FFEs on the proliferation, apoptosis, and antioxidant activity of yak GCs. An in vitro oxidative stress damage model was established by treating GCs treated with hydrogen peroxide (H₂O₂), and tested their response to exosomes. The results showed that exosomes pretreatment markedly inhibited the accumulation of reactive oxygen species (ROS), enhanced the activity of the antioxidant enzymes, such as SOD, CAT, and GSH, and up-regulated the mRNA and protein expression of the antioxidant stress-related genes, including SOD2, NFE2L2, GPX4, and CAT. In addition, exosome pretreatment increased the mRNA and protein levels of proliferation-related genes, PCNA, CDK4, CCND1, and CCNB1, and down-regulated the apoptosis-related genes, BAX, CASP3, and CASP8, in GCs. Our findings support the hypothesis that exosomes derived from follicular fluid can notably reduce oxidative stress and apoptosis in yak GCs. This study provides basic data for elucidating the molecular mechanism of exosomes action, proposes a novel avenue for regulating follicular development and atresia, and paves the way for future research into ways to improve yak breeding.

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来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.