Disturbances in cell wall biogenesis as a key factor in the replicative aging of budding yeast.

IF 4.4 4区 医学 Q1 GERIATRICS & GERONTOLOGY
Mateusz Mołoń, Gabriela Małek, Anna Bzducha-Wróbel, Monika Kula-Maximenko, Agnieszka Mołoń, Sabina Galiniak, Krzysztof Skrzypiec, Jacek Zebrowski
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引用次数: 0

Abstract

Aging is a multifactorial process that significantly impairs organismal function. Yeast is one of the model organisms used in aging research. Our understanding of the impact of the cell wall on aging remains elusive. Yeast cell wall is a complex and dynamic structure that plays a crucial role in the growth, survival, and aging of Saccharomyces cerevisiae. In this study, we demonstrated for the first time that the deletion of genes involved in cell wall biogenesis leads to significant impact on aging. In this study, we analysed five deletion mutants: crh2Δ, cwp1Δ, flo11Δ, gas1Δ and hsp12Δ. We showed a correlation between Raman spectroscopy signatures assigned to proteins, nucleic acids and RNA and replicative aging. Using Raman spectroscopy, we also revealed that a lack GAS1 gene results in significant changes in the biochemical composition of the cells that may increase sensitivity to environmental stressors. Our data unequivocally indicate that employing yeast as a model in aging research is appropriate, as long as the factors under analysis are not implicated in cell wall biogenesis.

衰老是一个多因素过程,会严重损害生物体的功能。酵母是衰老研究中使用的模式生物之一。我们对细胞壁对衰老的影响仍然缺乏了解。酵母细胞壁是一种复杂的动态结构,在酿酒酵母的生长、存活和衰老过程中起着至关重要的作用。在这项研究中,我们首次证明了删除参与细胞壁生物发生的基因会对衰老产生重大影响。在这项研究中,我们分析了五个基因缺失突变体:crh2Δ、cwp1Δ、flo11Δ、gas1Δ 和 hsp12Δ。我们发现蛋白质、核酸和 RNA 的拉曼光谱特征与复制老化之间存在相关性。通过拉曼光谱,我们还发现 GAS1 基因的缺失会导致细胞的生化成分发生显著变化,从而可能增加对环境压力的敏感性。我们的数据清楚地表明,只要所分析的因素与细胞壁的生物生成无关,采用酵母作为衰老研究的模型是合适的。
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来源期刊
Biogerontology
Biogerontology 医学-老年医学
CiteScore
8.00
自引率
4.40%
发文量
54
审稿时长
>12 weeks
期刊介绍: The journal Biogerontology offers a platform for research which aims primarily at achieving healthy old age accompanied by improved longevity. The focus is on efforts to understand, prevent, cure or minimize age-related impairments. Biogerontology provides a peer-reviewed forum for publishing original research data, new ideas and discussions on modulating the aging process by physical, chemical and biological means, including transgenic and knockout organisms; cell culture systems to develop new approaches and health care products for maintaining or recovering the lost biochemical functions; immunology, autoimmunity and infection in aging; vertebrates, invertebrates, micro-organisms and plants for experimental studies on genetic determinants of aging and longevity; biodemography and theoretical models linking aging and survival kinetics.
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