The Rhizospheric Alkali-Halotolerant Bacillus sp. KhSb-159 Enhanced Growth Parameters of the Mung Bean Crop.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-02-04 DOI:10.1007/s00284-025-04087-3

Nishtha R Vaghela, Sangeeta D Gohel

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Abstract

Plant growth-promoting rhizobacteria (PGPR) play a crucial role in enhancing plant growth through various direct and indirect mechanisms. In this investigation, we isolated a potential PGPR, Bacillus sp. KhSb-159, from the rhizosphere of Sorghum bicolor L. located at Kharaghoda, Little Rann of Kutch (LRK), Gujarat (India). The isolated Bacillus sp. KhSb-159 displayed distinctive characteristics, forming circular, sticky, opaque, moderate-sized colonies with brown pigmentation. Interestingly, this strain exhibited growth at an alkaline pH of 9, even in the presence of 1 M NaCl (w/v). Further analysis revealed its capacity to produce indole acetic acid (IAA), ammonia, siderophores, and the ability to solubilize phosphate. In addition, KhSb-159 showed extracellular enzyme secretion, including amylase, protease, and cellulase. Bacillus sp. strain KhSb-159 demonstrated its potential to enhance the growth of mung bean crops which is a significant agricultural application. The treated seeds exhibited improvements in various growth parameters such as shoot length, root length, plant biomass, number of leaves, number of roots, fresh weight of shoot, dry weight of shoot, fresh weight of root, dry weight of root, fresh weight of leaves, and dry weight of leaves. Overall, Bacillus sp. strain KhSb-159 would be a good choice for bioformulation in sustainable agriculture due to its diverse range of abilities. This study has implications for the advancement of eco-friendly farming methods and offers insightful information about using rhizobacterial strains to maximize plant growth.

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植物生长促进根瘤菌（PGPR）通过各种直接和间接机制在促进植物生长方面发挥着至关重要的作用。在这项研究中，我们从印度古吉拉特邦小卡奇沼泽地（LRK）卡拉戈达（Kharaghoda）高粱（Sorghum bicolor L.）的根瘤中分离出了一种潜在的 PGPR--芽孢杆菌 KhSb-159。分离出的芽孢杆菌 KhSb-159 表现出独特的特征，形成圆形、粘稠、不透明、中等大小、带有棕色色素的菌落。有趣的是，该菌株在 pH 值为 9 的碱性条件下也能生长，甚至在 1 M NaCl（w/v）的存在下也能生长。进一步分析表明，该菌株能产生吲哚乙酸（IAA）、氨、嗜苷酸，并能溶解磷酸盐。此外，KhSb-159 还能分泌胞外酶，包括淀粉酶、蛋白酶和纤维素酶。芽孢杆菌 KhSb-159 菌株证明了其促进绿豆作物生长的潜力，这是一项重要的农业应用。经处理的种子在各种生长参数上都有改善，如芽长、根长、植株生物量、叶片数、根数、芽鲜重、芽干重、根鲜重、根干重、叶片鲜重和叶片干重。总之，由于芽孢杆菌 KhSb-159 菌株具有多种能力，它将是可持续农业生物制剂的良好选择。这项研究对推进生态友好型农业方法具有重要意义，并为利用根瘤菌株最大限度地促进植物生长提供了深刻的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.