Reduced graphene oxide/gold composite synthesis via laser irradiation for surface enhanced Raman spectroscopy biosensors

IF 2.8 4区工程技术 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC

Journal of Materials Science: Materials in Electronics Pub Date : 2025-02-05 DOI:10.1007/s10854-025-14310-3

Alexander H. Xu, Liena Zaidan, Saeed Takaloo, Ilya Belyakov, Mehrdad Irannejad, Xenia Medvedeva, Sarah Labas, Bersu Bastug Azer, Ahmet Gulsaran, Dogu Ozyigit, Joel Pennings, Anna Klinkova, Reza Karimi, Joe Sanderson, Juewen Liu, Mustafa Yavuz

{"title":"Reduced graphene oxide/gold composite synthesis via laser irradiation for surface enhanced Raman spectroscopy biosensors","authors":"Alexander H. Xu, Liena Zaidan, Saeed Takaloo, Ilya Belyakov, Mehrdad Irannejad, Xenia Medvedeva, Sarah Labas, Bersu Bastug Azer, Ahmet Gulsaran, Dogu Ozyigit, Joel Pennings, Anna Klinkova, Reza Karimi, Joe Sanderson, Juewen Liu, Mustafa Yavuz","doi":"10.1007/s10854-025-14310-3","DOIUrl":null,"url":null,"abstract":"<div>Using SERS nanoplasmonic biosensors are attracting increasing interest due to their high sensitivity and non-destructive nature. Herein, we study the performance of reduced Graphene Oxide/Gold (rGO@AuNPs) biosensors, synthesized through a novel photoreduction method, for DNA detection applications. A parameter-controlled femtosecond laser approach has been proposed as a cost-effective, chemically stable, and suitable method for mass producing defect-free graphene sheets. Additionally, the laser-based rGO@AuNPs biosensor achieved an excellent enhancement factor and LOD of 105 and 10–7 M, respectively. SEM and TEM imaging were utilized to assess the morphology of rGO@AuNPs nanocomposites. Moreover, the optical properties of the nanocomposite before and after laser irradiation were studied using ultraviolet–visible spectroscopy, which showed a shift in peak wavelength from 228 to 268 nm after laser irradiation. Zeta potential measurements were conducted to analyze the ratio between GO and rGO, and it was found that use of lasers resulted in a reduction of 21% in GO oxygen content. As a proof of concept, the proposed biosensor was functionalized with 24-mer DNA to detect the complementary DNA probe at three concentrations 10–7, 10–6, and 10–5. This easy-synthesized advanced SERS nanoplasmonic biosensor is highly promising for early cancer diagnosis in the foreseeable future.</div>","PeriodicalId":646,"journal":{"name":"Journal of Materials Science: Materials in Electronics","volume":"36 4","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Materials Science: Materials in Electronics","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10854-025-14310-3","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}

引用次数: 0

Abstract

Using SERS nanoplasmonic biosensors are attracting increasing interest due to their high sensitivity and non-destructive nature. Herein, we study the performance of reduced Graphene Oxide/Gold (rGO@AuNPs) biosensors, synthesized through a novel photoreduction method, for DNA detection applications. A parameter-controlled femtosecond laser approach has been proposed as a cost-effective, chemically stable, and suitable method for mass producing defect-free graphene sheets. Additionally, the laser-based rGO@AuNPs biosensor achieved an excellent enhancement factor and LOD of 10⁵ and 10^–7 M, respectively. SEM and TEM imaging were utilized to assess the morphology of rGO@AuNPs nanocomposites. Moreover, the optical properties of the nanocomposite before and after laser irradiation were studied using ultraviolet–visible spectroscopy, which showed a shift in peak wavelength from 228 to 268 nm after laser irradiation. Zeta potential measurements were conducted to analyze the ratio between GO and rGO, and it was found that use of lasers resulted in a reduction of 21% in GO oxygen content. As a proof of concept, the proposed biosensor was functionalized with 24-mer DNA to detect the complementary DNA probe at three concentrations 10^–7, 10^–6, and 10^–5. This easy-synthesized advanced SERS nanoplasmonic biosensor is highly promising for early cancer diagnosis in the foreseeable future.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Materials Science: Materials in Electronics 工程技术-材料科学：综合

CiteScore

5.00

自引率

7.10%

发文量

1931

审稿时长

2 months

期刊介绍： The Journal of Materials Science: Materials in Electronics is an established refereed companion to the Journal of Materials Science. It publishes papers on materials and their applications in modern electronics, covering the ground between fundamental science, such as semiconductor physics, and work concerned specifically with applications. It explores the growth and preparation of new materials, as well as their processing, fabrication, bonding and encapsulation, together with the reliability, failure analysis, quality assurance and characterization related to the whole range of applications in electronics. The Journal presents papers in newly developing fields such as low dimensional structures and devices, optoelectronics including III-V compounds, glasses and linear/non-linear crystal materials and lasers, high Tc superconductors, conducting polymers, thick film materials and new contact technologies, as well as the established electronics device and circuit materials.