Wolbachia natural infection of mosquitoes in French Guiana: Prevalence, distribution, and genotyping

Médecine et Maladies Infectieuses Formation Pub Date : 2024-12-01 DOI:10.1016/j.mmifmc.2024.11.048

E. Clervil , A. Guidez , S. Talaga , R. Carinci , P. Gaborit , A. Lavergne , S. Tirera , J.-B. Duchemin

{"title":"Wolbachia natural infection of mosquitoes in French Guiana: Prevalence, distribution, and genotyping","authors":"E. Clervil , A. Guidez , S. Talaga , R. Carinci , P. Gaborit , A. Lavergne , S. Tirera , J.-B. Duchemin","doi":"10.1016/j.mmifmc.2024.11.048","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div><em>Wolbachia</em> are the most spread bacterial endosymbionts in the world. These bacteria can manipulate the host reproduction or block virus transmission in mosquitoes. For this reason, <em>Wolbachia</em> based strategies for vector control is seriously considered or already applied in several countries in the world. In South America, <em>Wolbachia</em> have been studied in human pathogen vectors such as sand flies and mosquitoes. In French Guiana, the diversity and distribution of <em>Wolbachia</em> are not well known in mosquitoes.</div></div><div><h3>Methods</h3><div>In this study, we screened for <em>Wolbachia</em> natural infection of mosquitoes in French Guiana by 16S rRNA, <em>Wolbachia</em> Surface Protein (WSP) gene and Multilocus Sequence Typing (MLST) molecular assays. Twenty-three out of 44 (52%) mosquito species were found positive for natural <em>Wolbachia</em> infection by PCR and highlights a two strains <em>Wolbachia</em> co-infection in three specimens of <em>Mansonia titillans</em>. Then, we analysed the phylogenetic relationships among the <em>Wolbachia</em> detected.</div></div><div><h3>Results</h3><div>All tested specimens of <em>Aedes aegypti</em>, the major dengue vector of French Guiana, were found negative. These results about <em>Wolbachia</em> strains, distribution, and prevalence in mosquitoes of French Guiana pave the way to future <em>Wolbachia</em> based strategy for vector control in this amazonian territory.</div></div>","PeriodicalId":100906,"journal":{"name":"Médecine et Maladies Infectieuses Formation","volume":"3 4","pages":"Page S23"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Médecine et Maladies Infectieuses Formation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772743224006317","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction

Wolbachia are the most spread bacterial endosymbionts in the world. These bacteria can manipulate the host reproduction or block virus transmission in mosquitoes. For this reason, Wolbachia based strategies for vector control is seriously considered or already applied in several countries in the world. In South America, Wolbachia have been studied in human pathogen vectors such as sand flies and mosquitoes. In French Guiana, the diversity and distribution of Wolbachia are not well known in mosquitoes.

Methods

In this study, we screened for Wolbachia natural infection of mosquitoes in French Guiana by 16S rRNA, Wolbachia Surface Protein (WSP) gene and Multilocus Sequence Typing (MLST) molecular assays. Twenty-three out of 44 (52%) mosquito species were found positive for natural Wolbachia infection by PCR and highlights a two strains Wolbachia co-infection in three specimens of Mansonia titillans. Then, we analysed the phylogenetic relationships among the Wolbachia detected.

Results

All tested specimens of Aedes aegypti, the major dengue vector of French Guiana, were found negative. These results about Wolbachia strains, distribution, and prevalence in mosquitoes of French Guiana pave the way to future Wolbachia based strategy for vector control in this amazonian territory.

查看原文本刊更多论文

法属圭亚那蚊子沃尔巴克氏体自然感染：流行、分布和基因分型

沃尔巴克氏体是世界上传播最广的细菌内共生菌。这些细菌可以控制宿主的繁殖或阻断病毒在蚊子中的传播。因此，世界上一些国家正在认真考虑或已经采用以沃尔巴克氏体为基础的病媒控制战略。在南美洲，研究人员在人类病原体媒介如沙蝇和蚊子中发现了沃尔巴克氏体。在法属圭亚那，沃尔巴克氏体在蚊子中的多样性和分布并不为人所知。方法采用16S rRNA、沃尔巴克氏体表面蛋白（Wolbachia Surface Protein， WSP）基因和多位点序列分型（Multilocus Sequence Typing， MLST）方法对法属圭亚那蚊虫沃尔巴克氏体自然感染进行筛选。44种蚊中有23种（52%）自然沃尔巴克氏体感染阳性，其中3种曼陀罗蚊标本中发现两株沃尔巴克氏体共感染。然后，我们分析了检测到的沃尔巴克氏体的系统发育关系。结果法属圭亚那主要登革热媒介埃及伊蚊检测结果均为阴性。这些关于法属圭亚那沃尔巴克氏体菌株、分布和流行情况的结果为今后在该亚马逊地区采用基于沃尔巴克氏体的病媒控制策略铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Médecine et Maladies Infectieuses Formation

自引率

0.00%

发文量