Co-administration of Hibiscus sabdariffa linn and Daflon-500® mitigates weight loss, hyperglycemia, hematological and oxidative changes in male rats with type-2 diabetes

Q3 Pharmacology, Toxicology and Pharmaceutics

Phytomedicine Plus Pub Date : 2025-02-01 DOI:10.1016/j.phyplu.2024.100699

Aishat Omobolanle Olatunji , Root Oluwapelumi Alegbe , Suleiman Folorunsho Ambali , Muftau Shittu , Ganiu Jimoh Akorede , Abdulfatai Aremu , Afisu Basiru , Jamila Abdulhamid Atata , Kolawole Yusuf Suleiman , Charles Amoto Shuaibu

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Abstract

Background

Diabetes mellitus is a complex metabolic disorder associated with persistent hyperglycemia and metabolic imbalance. Flavonoid compounds possess notable qualities in mitigating diabetes and its complications.

Aim

Hibiscus sabdariffa linn and daflon-500® were assessed for their effects on body weight, blood glucose/indices, and oxidative changes in type-2 diabetes rats over 28 days.

Methods

35 male rats were randomly divided into seven groups (n = 5). Diabetes mellitus was induced in groups 2–7 via a single intraperitoneal injection of streptozotocin (STZ; 55mg/kg). Group 1(DW) received distilled water (2mL/kg) only. Group 2 (STZ; untreated), group 3 {MET; metformin (Glucophage)}, group 4 {HS; Hibiscus sabdariffa (400mg/kg)}, group 5 {DAF; daflon-500® (1000mg/kg)}, group 6 {DAF+HS; daflon-500® (1000mg/kg) and Hibiscus sabdariffa (400mg/kg)}, group 7 {DAF+MET+HS; daflon-500® (1000mg/kg), metformin (Glucophage), and Hibiscus sabdariffa (400mg/kg)}. Body weight and blood glucose were assessed fortnightly. Blood and hepatic/pancreatic tissues were collected for hematological and antioxidant testing.

Results

Groups 2–7 revealed a significant (P < 0.05) decrease in body weight on day 14 compared to the increased growth rate observed on day 28 (Group 7).

Blood glucose levels significantly increased on days 1 and 14 in groups 2–7 but declined on day 28 in groups 4–7. Significantly higher packed cell volume, hemoglobin levels, red blood cell counts, lymphocytes, and mean corpuscular hemoglobin were observed in groups 5–7. Platelet counts were higher in Groups 2 and 5. Untreated group 2 exhibited considerably reduced catalase activity, glutathione, and malondialdehyde concentrations compared to the treated groups.

Conclusion

Hibiscus sabdariffa linn and Daflon-500® expressed substantial hypoglycemic, antioxidant, and hemato-protective effects.

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同时给药芙蓉和达芙蓉-500®可减轻2型糖尿病雄性大鼠的体重减轻、高血糖、血液学和氧化变化

背景：糖尿病是一种复杂的代谢紊乱，伴有持续性高血糖和代谢失衡。黄酮类化合物在减轻糖尿病及其并发症方面具有显著的品质。我们在28天内评估了芙蓉和达芙蓉-500®对2型糖尿病大鼠的体重、血糖/指数和氧化变化的影响。方法35只雄性大鼠随机分为7组（n = 5）， 2 ~ 7组采用单次腹腔注射链脲佐菌素(STZ；55毫克/公斤)。第一组（DW）仅给予蒸馏水（2mL/kg）。第二组(STZ；未经处理)，第3组{MET；二甲双胍（Glucophage）}，第4组{HS；芙蓉（400mg/kg）}，第5组{DAF；dafron -500®（1000mg/kg）}，第6组{DAF+HS；达芙蓉-500®（1000mg/kg）和芙蓉（400mg/kg）}， 7组{DAF+MET+HS；达芙蓉-500®（1000mg/kg）、二甲双胍（Glucophage）和芙蓉（400mg/kg）}。每两周评估体重和血糖。采集血液和肝/胰腺组织进行血液学和抗氧化检测。结果2-7组患者P <；与第28天的生长率升高相比，第14天体重降低（0.05）（第7组）。第2-7组的血糖水平在第1天和第14天显著升高，而第4-7组的血糖水平在第28天下降。5 ~ 7组的堆积细胞体积、血红蛋白水平、红细胞计数、淋巴细胞和平均红细胞血红蛋白均显著升高。第2组和第5组血小板计数较高。与治疗组相比，未治疗组2表现出显著降低的过氧化氢酶活性、谷胱甘肽和丙二醛浓度。结论芙蓉和达芙蓉-500具有明显的降血糖、抗氧化和血液保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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