Co-administration of Hibiscus sabdariffa linn and Daflon-500® mitigates weight loss, hyperglycemia, hematological and oxidative changes in male rats with type-2 diabetes

Q3 Pharmacology, Toxicology and Pharmaceutics

Phytomedicine Plus Pub Date : 2025-02-01 DOI:10.1016/j.phyplu.2024.100699

Aishat Omobolanle Olatunji , Root Oluwapelumi Alegbe , Suleiman Folorunsho Ambali , Muftau Shittu , Ganiu Jimoh Akorede , Abdulfatai Aremu , Afisu Basiru , Jamila Abdulhamid Atata , Kolawole Yusuf Suleiman , Charles Amoto Shuaibu

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Abstract

Background

Diabetes mellitus is a complex metabolic disorder associated with persistent hyperglycemia and metabolic imbalance. Flavonoid compounds possess notable qualities in mitigating diabetes and its complications.

Aim

Hibiscus sabdariffa linn and daflon-500® were assessed for their effects on body weight, blood glucose/indices, and oxidative changes in type-2 diabetes rats over 28 days.

Methods

35 male rats were randomly divided into seven groups (n = 5). Diabetes mellitus was induced in groups 2–7 via a single intraperitoneal injection of streptozotocin (STZ; 55mg/kg). Group 1(DW) received distilled water (2mL/kg) only. Group 2 (STZ; untreated), group 3 {MET; metformin (Glucophage)}, group 4 {HS; Hibiscus sabdariffa (400mg/kg)}, group 5 {DAF; daflon-500® (1000mg/kg)}, group 6 {DAF+HS; daflon-500® (1000mg/kg) and Hibiscus sabdariffa (400mg/kg)}, group 7 {DAF+MET+HS; daflon-500® (1000mg/kg), metformin (Glucophage), and Hibiscus sabdariffa (400mg/kg)}. Body weight and blood glucose were assessed fortnightly. Blood and hepatic/pancreatic tissues were collected for hematological and antioxidant testing.

Results

Groups 2–7 revealed a significant (P < 0.05) decrease in body weight on day 14 compared to the increased growth rate observed on day 28 (Group 7).

Blood glucose levels significantly increased on days 1 and 14 in groups 2–7 but declined on day 28 in groups 4–7. Significantly higher packed cell volume, hemoglobin levels, red blood cell counts, lymphocytes, and mean corpuscular hemoglobin were observed in groups 5–7. Platelet counts were higher in Groups 2 and 5. Untreated group 2 exhibited considerably reduced catalase activity, glutathione, and malondialdehyde concentrations compared to the treated groups.