Kartogenin-mediated transformation of osteo-chondroprogenitors from stem cells facilitates bone repair via intramembranous and endochondral ossification

IF 12.7 1区材料科学 Q1 ENGINEERING, MULTIDISCIPLINARY

Composites Part B: Engineering Pub Date : 2025-01-21 DOI:10.1016/j.compositesb.2025.112161

Chengyuan Liu , Huan Wang , Ye Gu , Zhangqin Yuan , Qifan Yu , Changjiang Liu , Han Sun , Yuanchen Zhu , Qianping Guo , Caihong Zhu , Bin Li

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引用次数: 0

Abstract

Bone tissue engineering through regulation of mesenchymal stem cells (MSCs) provides a promising strategy for bone repair via intramembranous or endochondral ossification. Here, the novel approach of promoting the transformation of MSCs into osteo-chondroprogenitors to simulate the initial process in bone development to improve bone repair was proposed and validated via the use of a small-molecule drug, kartogenin (KGN). The transformation of MSCs into osteo-chondroprogenitors was effectively achieved by KGN treatment in 1 week. In the Ca²⁺-rich bone microenvironment in vitro, the transformation of MSCs markedly enhanced osteoblast generation by upregulating SOX9, RUNX2, and β-catenin. In vivo experiments further verified that the transformation of osteo-chondroprogenitors drove rapid bone repair within 4 weeks, through both intramembranous and endochondral ossification. This study proposes accelerating bone regeneration by inducing the transformation of MSCs into osteo-chondroprogenitors and reveals the co-existence of intramembranous and endochondral ossification during bone repair, providing new insights for bone regeneration.

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来源期刊

Composites Part B: Engineering 工程技术-材料科学：复合

CiteScore

24.40

自引率

11.50%

发文量

784

审稿时长

21 days

期刊介绍： Composites Part B: Engineering is a journal that publishes impactful research of high quality on composite materials. This research is supported by fundamental mechanics and materials science and engineering approaches. The targeted research can cover a wide range of length scales, ranging from nano to micro and meso, and even to the full product and structure level. The journal specifically focuses on engineering applications that involve high performance composites. These applications can range from low volume and high cost to high volume and low cost composite development. The main goal of the journal is to provide a platform for the prompt publication of original and high quality research. The emphasis is on design, development, modeling, validation, and manufacturing of engineering details and concepts. The journal welcomes both basic research papers and proposals for review articles. Authors are encouraged to address challenges across various application areas. These areas include, but are not limited to, aerospace, automotive, and other surface transportation. The journal also covers energy-related applications, with a focus on renewable energy. Other application areas include infrastructure, off-shore and maritime projects, health care technology, and recreational products.