A novel aptamer-based probe for the identification and enrichment of trophoblast cells

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B Pub Date : 2025-01-29 DOI:10.1016/j.jchromb.2025.124479

Mang Gui , Weiwei Xu , Yueyue Gui , Chenyun Zhang , Dandan Shi , Jiehua Ma , Jin Qiu

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引用次数: 0

Abstract

Trophoblast cells, which are shed into the maternal reproductive tract, are emerging as a valuable source for prenatal diagnostic cells. However, the current enrichment efficiency of these cells is inadequate for clinical purposes. The reliance on antibody-based cell recognition methods has been a significant barrier to developing more effective enrichment techniques. In our research, we employed systematic evolution of ligands by exponential enrichment (SELEX) technology to identify three aptamers that can specifically bind to the HLA-G protein, a unique marker on the surface of trophoblast cells. Through a process of meticulous truncation and optimization, we successfully developed the highly effective nucleic acid aptamer H4–2. Utilizing H4–2, we engineered a novel circulating microfluidic system that achieves an impressive 65 % enrichment efficiency for trophoblast cells. This advancement holds substantial promise for enhancing prenatal diagnostic methods that rely on intact foetal cells.

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.