Performance and phosphorus release of an anaerobic co-digestion of excess sludge and food waste containing garlic at varying substrate ratio

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-01-29 DOI:10.1016/j.bej.2025.109659

Lixin Wei , Yuchen Wang , Yuan He , Guangrong Zhou , Shutong Dong , Yongan Chen , Qing Wang , Yuxiang Lu , Chengyuan Su

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Abstract

The effects of different ratios of food waste (FW) containing garlic and excess sludge (ES) on the anaerobic co-digestion (AcoD) process were investigated. This was done by establishing a sequencing batch anaerobic digester and analyzing phosphorus release patterns, microbial community structure and gene function in the AcoD system with 1:1, 1:2, 1:4, and 1:8 ratio of FW to ES. The highest phosphine (PH₃) gas output of 1619.99 mg/m³ was observed when FW:ES was 1:1. The relative abundance of Methanothrix increased from 7.99 % to 9.36 % with variation the ratio of FW:ES from 1:4–1:8. The contents of phnD and phnE genes in the ratio of 1:4 FW:ES group were the highest among all experimental groups, with 0.0010 % and 0.0018 %, respectively. In addition, the abundance of functional genes responsible for encoding acetate kinase in the 1:4 FW:ES group increased by 152.96 % relative to the control group. Tests determined the optimal FW to ES ratios to achieve 1:4 and 1:8, and provided suitable operating conditions and references for the subsequent AcoD research.

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不同底物比下含大蒜的剩余污泥和食物垃圾厌氧共消化的性能和磷释放

研究了不同比例含蒜食物垃圾（FW）与剩余污泥（ES）对厌氧共消化（AcoD）过程的影响。通过建立序批式厌氧消化池，分析了FW与ES比例分别为1:1、1:2、1:4和1:8的AcoD系统中磷的释放规律、微生物群落结构和基因功能。当FW:ES为1:1时，磷化氢（PH3）气体产量最高，为1619.99 mg/m3。甲烷菌的相对丰度由7.99 %增加到9.36 %，FW:ES比值由1:4 ~ 1:8变化。FW:ES比例为1:4组phnD和phnE基因含量最高，分别为0.0010 %和0.0018 %。此外，与对照组相比，1:4 FW:ES组编码乙酸激酶的功能基因丰度增加了152.96 %。试验确定了最佳的FW / ES比为1:4和1:8，为后续的AcoD研究提供了合适的操作条件和参考。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.