A Golgi-localized fluorescence probe for measuring cysteine based on a naphthalimide derivative

IF 4.1 3区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Photochemistry and Photobiology A-chemistry Pub Date : 2025-01-16 DOI:10.1016/j.jphotochem.2025.116276

Qiujuan Ma , Junhong Xu , Shuzhen Liu , Guojiang Mao , Shuqi Hou , Yijie Ma , Yujie Lian

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Abstract

Cysteine is an essential thiol-containing amino acid in biological systems and is involved in several physiological processes in the human body. The Golgi apparatus is the main site of protein processing, and the normal concentration of cysteine is an important factor to maintain the homeostasis in Golgi apparatus. Once the Golgi apparatus homeostasis is disrupted, it will lead to the Golgi apparatus dysfunction, which will lead to a series of diseases. Herein a new Golgi-localized fluorescent probe was constructed for measuring cysteine. The developed probe was assembled by utilizing a 1,8-naphthalimide derivative as the fluorescent component, appending a phenylsulfonamide group to target the Golgi apparatus, and incorporating an acrylate recognition unit to interact with and detect cysteine. Within the concentration range of 2.0 × 10⁻⁷–1.0 × 10⁻⁵ mol·L⁻¹, a favorable linear correlation was observed between the fluorescence intensity of the probe at 550 nm and the cysteine concentration. Furthermore, the detection limit was determined to be 1.56 × 10⁻⁸ mol·L⁻¹. The proposed probe exhibited superior selectivity, swift response time, and wide pH operational scope. In addition, the proposed probe was almost non-cytotoxic and was able to localize to the Golgi apparatus and detect endogenous and exogenous cysteine in A549 cells.

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来源期刊

Journal of Photochemistry and Photobiology A-chemistry 化学-物理化学

CiteScore

7.90

自引率

7.00%

发文量

580

审稿时长

48 days

期刊介绍： JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.