Exploring the recognition behavior of a fluorescein-based probe towards the significant detection of Cu2+ and Zn2+ ions: Experimental and computational studies

IF 4.1 3区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Photochemistry and Photobiology A-chemistry Pub Date : 2025-01-10 DOI:10.1016/j.jphotochem.2025.116268

Navneet Kumar , Pawan Kumar Sada , Amit Kumar Kundan , Amit Bar , Amanpreet Kaur Jassal , Surya Prakash Rai , Vipendra Kumar Singh , Neha Garg , Alok Kumar Singh , Ankit Kumar Singh , Sumit Kumar , Laxman Singh , Abhishek Rai

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引用次数: 0

Abstract

A new fluorescein hydrazone (FA1) has been synthesized employing fluorescein hydrazide and 3,5-diiodosalicylaldehyde and its complete physiochemical characterization have been carried out. A single crystal XRD studies of FA1 has been performed. Among the tested metal ions FA1 significantly detects Cu²⁺ and Zn²⁺ in EtOH/H₂O (8:2, v/v)). There is an emergence of a new absorbance peak at λ_max 431 nm with Cu²⁺ ions. A “turn-on” behavior in fluorescence at λ_em510 nm was observed with Zn²⁺ ions owing to chelation enhanced fluorescence. This act of spectrophotometric changes and naked eye color variation from colorless to yellow is because of coordination with the metal ions rather than most common opening of spirolactum ring. FA1-Cu²⁺ and FA1-Zn²⁺ensembles display reversible behavior with EDTA²⁻ ions. In a study on latent fingerprint detection using powder compounds, it was found that FA1 and FA1–Zn²⁺ showed excellent adherence to finger ridges and produced clear features without any background stains. The Hirshfeld surface and fingerprint analysis of FA1 offer a detailed examination of pairwise interactions between atoms. Additionally, topological analysis of FA1 has been conducted using NCI, AIM, ELF, and LOL methodologies. Alamar assay of FA1 on HEK-293 cell lines exhibited biocompatibility with minimal cytotoxicity. Hence, they were effectively applied for live cell imaging for intracellular sensing of Cu²⁺ and Zn²⁺ in HEK-293 cells.

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来源期刊

Journal of Photochemistry and Photobiology A-chemistry 化学-物理化学

CiteScore

7.90

自引率

7.00%

发文量

580

审稿时长

48 days

期刊介绍： JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.