Nondestructive three-dimensional structure and growth characteristics of anammox granular sludge

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation Pub Date : 2025-02-01 DOI:10.1016/j.ibiod.2024.105986

Meng-Wen Peng , Ya-Song Chen , You-Peng Chen , Wan Sun , Meng-Meng Liu , Xun Weng , Shuai Wang , Yu Shen , Hui-Min Fu

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引用次数: 0

Abstract

The three-dimensional (3D) structure of Anaerobic ammonium oxidation (Anammox) granular sludge (AnGS) is vital for substrate transfer and metabolite excretion, yet its intricacies are not fully understood due to a lack of in situ non-destructive characterization methods. This study employs synchrotron-based X-ray micro-computed tomography to visualize the 3D structure of AnGS at different growth stages. We observed that porosity and pore size increased with granule depth, while larger granules displayed reduced porosity (decreased from 85.3% to 63.1%) and more concentrated pore structures. Medium-sized granules (4.2–5.0 mm) exhibited the highest abundance of Anammox bacteria. Our findings revealed significant correlations among pore structure, specific anammox activity, and microbial community, particularly with Candidatus Jettenia and Norank_f_SJA-28. This study offers a comprehensive understanding of the 3D structure and growth characteristics of AnGS, providing theoretical support for substrate mass transfer and enhancing nitrogen removal efficiency within these systems.

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厌氧氨氧化颗粒污泥无损三维结构及生长特性研究

厌氧氨氧化（Anammox）颗粒污泥（AnGS）的三维（3D）结构对于底物转移和代谢物排泄至关重要，但由于缺乏原位无损表征方法，其复杂性尚未完全了解。本研究采用基于同步加速器的x射线微计算机断层扫描技术来观察不同生长阶段AnGS的三维结构。我们观察到孔隙度和孔径随颗粒深度的增加而增加，颗粒越大孔隙度越低（从85.3%下降到63.1%），孔隙结构越集中。中型颗粒（4.2 ~ 5.0 mm）厌氧氨氧化菌丰度最高。我们的研究结果揭示了孔隙结构、厌氧氨氧化活性和微生物群落之间的显著相关性，特别是与候选菌Jettenia和Norank_f_SJA-28之间的相关性。该研究对AnGS的三维结构和生长特性有了全面的了解，为这些系统的底物传质和提高脱氮效率提供了理论支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Biodeterioration & Biodegradation 生物-环境科学

CiteScore

9.60

自引率

10.40%

发文量

107

审稿时长

21 days

期刊介绍： International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.