A label-free gold nanoparticles functionalized peptide dendrimer biosensor for visual detection of breakthrough infections in COVID-19 vaccinated patients

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI:10.1016/j.sbsr.2024.100718

Naveen Kumar , Ashutosh Singh , Preeti Dhaka , Ankur Singh , Pragya Agarwala , Kuldeep Sharma , Anudita Bhargava , Sandeep Bhatia , Thomas Launey , Rahul Kaushik , Shailly Tomar , Aniket Sanyal

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Abstract

Given the global implementation of effective COVID-19 vaccines, which do not confer complete immunity, it is crucial to monitor the occurrence of breakthrough infections, particularly against newly emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants. Hence, we developed a label-free colorimetric assay using gold nanoparticles (GNPs) functionalized with a peptide dendrimer incorporating highly reactive epitopes of the nucleocapsid (N) protein. This assay relies on the tween-20 induced colorimetric changes caused by the aggregation of peptide dendrimer-coated GNPs in the absence of anti- SARS-CoV-2 N antibodies, and vice versa. Transmission electron microscopy, dynamic light scattering, and circular dichroism spectroscopy analyses all showed the formation of a uniform and highly stable coating of the peptide dendrimer over GNPs. Surface plasmon resonance experiments have demonstrated a strong binding affinity for the peptide dendrimer and anti- SARS-CoV-2 N antibodies, with a K_D value of 525 nM. To validate the proof-of-concept, we have tested this assay on seventy human serum samples, and receiver operating characteristic curve analysis demonstrated high diagnostic sensitivity (88.89 %) and specificity (100 %). This approach opens up new avenues for the development of simple and rapid diagnostic assays for identifying antibodies against viral infections and other pathogens.

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来源期刊

Sensing and Bio-Sensing Research Engineering-Electrical and Electronic Engineering

CiteScore

10.70

自引率

3.80%

发文量

审稿时长

87 days

期刊介绍： Sensing and Bio-Sensing Research is an open access journal dedicated to the research, design, development, and application of bio-sensing and sensing technologies. The editors will accept research papers, reviews, field trials, and validation studies that are of significant relevance. These submissions should describe new concepts, enhance understanding of the field, or offer insights into the practical application, manufacturing, and commercialization of bio-sensing and sensing technologies. The journal covers a wide range of topics, including sensing principles and mechanisms, new materials development for transducers and recognition components, fabrication technology, and various types of sensors such as optical, electrochemical, mass-sensitive, gas, biosensors, and more. It also includes environmental, process control, and biomedical applications, signal processing, chemometrics, optoelectronic, mechanical, thermal, and magnetic sensors, as well as interface electronics. Additionally, it covers sensor systems and applications, µTAS (Micro Total Analysis Systems), development of solid-state devices for transducing physical signals, and analytical devices incorporating biological materials.