Synthesis of size-controlled poly(vinyldiaminotriazine) nanoparticles for enhanced hydrogen bonding adsorption of horseradish peroxidase

IF 4.5 3区工程技术 Q1 CHEMISTRY, APPLIED

Reactive & Functional Polymers Pub Date : 2025-01-12 DOI:10.1016/j.reactfunctpolym.2025.106156

Xiaotao Wang , Chuan Xu , Feiyang Xiao , Xiangning Yan , Chak-Yin Tang , Huiling Guo , Wing-Cheung Law

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Abstract

Physically adsorbing proteins through functional polymeric nanoparticles hold great potential for a variety of applications. Achieving strong and stable adsorption via hydrogen bonding in aqueous phases is challenging due to the interference from water molecules. The monomer 2-vinyl-4,6-diamino-1,3,5-triazine (VDAT) not only provides donor and receptor sites for hydrogen bonding but also possesses an apolar nature that can help prevent water molecules from interfering with these bonding sites. In this work, poly(vinyldiaminotriazine) nanoparticles (PVDAT) with sizes ranging from approximately 50 nm to 240 nm were synthesized through semi-continuous precipitation polymerization in water. The formation mechanism of PVDAT, including particle nucleation and growth stages, was investigated. VDAT oligomers aggregated to form a core and subsequently grew by adsorbing additional VDAT oligomers. Adsorption studies of PVDAT on horseradish peroxidase (HRP) demonstrated stable physical adsorption facilitated by hydrogen bonding between PVDAT and the enzyme in the aqueous phase. The adsorption of HRP by PVDAT followed the Langmuir model of single-layer adsorption, with a maximum adsorption capacity of 13.80 mg/g and a retention of enzymatic activity of ∼74.99 %. This innovative approach aims to enhance the precision and efficacy of protein separation and extraction, as well as the efficiency of enzyme immobilization.

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来源期刊

Reactive & Functional Polymers 工程技术-高分子科学

CiteScore

8.90

自引率

5.90%

发文量

259

审稿时长

27 days

期刊介绍： Reactive & Functional Polymers provides a forum to disseminate original ideas, concepts and developments in the science and technology of polymers with functional groups, which impart specific chemical reactivity or physical, chemical, structural, biological, and pharmacological functionality. The scope covers organic polymers, acting for instance as reagents, catalysts, templates, ion-exchangers, selective sorbents, chelating or antimicrobial agents, drug carriers, sensors, membranes, and hydrogels. This also includes reactive cross-linkable prepolymers and high-performance thermosetting polymers, natural or degradable polymers, conducting polymers, and porous polymers. Original research articles must contain thorough molecular and material characterization data on synthesis of the above polymers in combination with their applications. Applications include but are not limited to catalysis, water or effluent treatment, separations and recovery, electronics and information storage, energy conversion, encapsulation, or adhesion.

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阿拉丁

2,2′-azobis (2-methylpropionamidine) dihydrochloride