An innovative method for sensitive spectrophotometric estimation of doxorubicin hydrochloride in injections and biological fluids by complex formation with dysprosium

Abstract

Doxorubicin hydrochloride is a very important chemical drug widely used in the treatment of number of cancerous diseases. So, it is important to monitor doxorubicin hydrochloride levels during treatment and determine its quantity due to its fatal effect on the heart muscle related to the amount of doses given to a patient. This study adopted a new, simple, rapid, and sensitive spectrophotometric method to determine doxorubicin hydrochloride in an aqueous medium. The method relies on the ability of doxorubicin hydrochloride to form a water-soluble complex with trivalent dysprosium ion. The influences of pH, time, dysprosium quantity, linearity of the calibration curve, temperature and stoichiometric ratio of the product were studied under ideal conditions for the formation of the pink complex between doxorubicin hydrochloride and dysprosium ion. The wavelength in which the compound exhibited the greatest absorption was 534 nm. Beer's law was followed by the calibration curve's linearity in the 0.5–43 μg/L range, with a correlation coefficient of 0.9988. The quantification limit is 0.0352 μg/L, and the detection limit is 0.0112. The relative standard deviation of four replicates was identified to range from 0.032 % to 0.388 %, contingent upon the concentration level of doxorubicin hydrochloride in the sample. In contrast, the accuracy was found to be between 96.33 % and 101.6 %, unaffected by any common excipients present in the samples. The t-test and F-test variables at a 95 % confidence level were analyzed, revealing no significant difference between the recommended and reference techniques.The proposed method has been successfully applied for the determination of doxorubicin hydrochloride in diverse specimens of doxorubicin including pharmaceutical injections and biological fluids (urine and serum).