Phytochemical analysis and investigation of antioxidant and anti-inflammatory activities of selected Ethiopian traditional medicinal plants

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine Pub Date : 2025-02-01 DOI:10.1016/j.hermed.2024.100984

Juhar Zemede , Belay Melese , Tegenu Mekuria , Gui-Lin Chen , Guang-Wan Hu

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Abstract

Introduction

Research on bioactive compound isolations, descriptions, and biological tests on medicinal plants is still limited. This study primarily explores the secondary bioactive metabolites and the pharmacological potential of four medicinal plants from Ethiopia. The study aimed to screen phytochemical constituents and investigate their antioxidant and anti-inflammatory potential.

Methods

The ethanolic extracts of Clerodendrum myricoides (Hochst.) R.Br. ex Vatke, Millettia ferruginea (Hochst.) Hochst. ex Baker, Vepris dainellii (Pic.Serm.) Kokwaro, and Fuerstia africana T.C.E.Fr. r. leaves were subjected to Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UPLC-QTOF-MS)/MS for phytochemical analysis and assays, including anti-oxidant (2,2-diphenyl-1-picrylhydrazyl [DPPH], ferric reducing-ion reducing power [FRAP]), Cyclooxygenase 2 (COX-2) inhibition for anti-inflammatory, and Folin–Ciocalteu for phenolic contents determination.

Results

The total phenolic contents of C. myricoides, M. Ferruginea, V. dainellii, and F. africana were 125.68 ± 0.82 mg, 54.97 ± 0.94, 113.57 ± 0.99 and 66.40 ±1.14 mg GAE/g, while flavonoid contents were 236.25 ± 1.85, 78.12 ± 0.96, 51.148 ± 1.09 and 60.79 ± 0.47 mg RE/g, respectively. C. myricoides and F. africana have higher DPPH scavenging rates with IC₅₀: 1.10 ± 0.12, 2.09 ± 0.14, mg/ml. C. myricoides and F. africana displayed the highest FRAP values with 33.30 ± 0.92 and 23.87 ± 1.42 mM Fe²⁺/g. F. africana exerted highest anti-inflammatory activities with COX-2 inhibition at IC₅₀ of 1.82 ± 0.04 μg/ml. About 61 bioactive metabolites are identified. Alkaloids, phenolic, flavonoids, and terpenoids were the dominant class of identified compounds.

Conclusions

The pharmacological tests have validated the therapeutic uses of traditional medicinal plants. Bioactive metabolites play a crucial role in the potential therapeutic applications of plants.

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埃塞俄比亚传统药用植物抗氧化和抗炎活性的植物化学分析和研究

药用植物生物活性化合物的分离、描述和生物学试验的研究仍然有限。本研究主要探讨了埃塞俄比亚四种药用植物的次生生物活性代谢物及其药理潜力。本研究旨在筛选植物化学成分并研究其抗氧化和抗炎潜能。方法：提取杨梅藤的乙醇提取物。R.Br。原Vatke， Millettia ferruginea （Hochst.）Hochst。前Baker， Vepris dainellii（图. serm）Kokwaro和Fuerstia africana T.C.E.Fr。采用超高效液相色谱-四极杆飞行时间质谱(UPLC-QTOF-MS)/MS进行植物化学分析和测定，包括抗氧化（2,2-二苯基-1-苦基肼[DPPH]）、铁还原离子还原力（FRAP]）、抗炎抑制环氧化酶2 （COX-2）和福林- ciocalteu测定酚类含量。结果杨梅、铁锈、丹ellii和非洲麻黄总酚含量分别为125.68±0.82 mg、54.97±0.94、113.57±0.99和66.40±1.14 mg RE/g，黄酮类含量分别为236.25±1.85、78.12±0.96、51.148±1.09和60.79±0.47 mg RE/g。C. myricoides和F. africana对DPPH的清除率较高，IC50分别为1.10±0.12,2.09±0.14,mg/ml。myricoides和F. africana的FRAP值最高，分别为33.30±0.92和23.87±1.42 mM Fe2+/g。对COX-2抑制的IC50值为1.82±0.04 μg/ml，非洲菌的抗炎活性最高。鉴定出约61种生物活性代谢物。生物碱、酚类、黄酮类和萜类是鉴定出的主要化合物。结论药理学试验证实了传统药用植物的治疗作用。生物活性代谢物在植物的潜在治疗应用中起着至关重要的作用。

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来源期刊

Journal of Herbal Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.