Exploiting in vitro gastric acid-neutralising synergy between Desmodium triflorum (L.) DC. and Pogostemon heyneanus Benth. in an antacid tablet formulation

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine Pub Date : 2025-02-01 DOI:10.1016/j.hermed.2024.100981

Bhavantha Dias , Banukie Jayasuriya , Dilanthi Herath , Menuka Arawwawala

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引用次数: 0

Abstract

Introduction

This study aimed to evaluate the combinative in vitro antacid activity of two terrestrial medicinal plants; Desmodium triflorum and Pogostemon heyneanus, and to subsequently formulate an antacid dosage form using their extracts.

Methods

The gastric acid-neutralising activity of crude aqueous extract combinations of D. triflorum whole plant and P. heyneanus leaves was evaluated using different in vitro assays, against distilled water. Then, the 3:1 concentration combination of D. triflorum and P. heyneanus was membrane dialysed and the resulting fractions were assayed in Vatier’s artificial stomach model. The same extract combination was characterised by high-performance liquid chromatography (HPLC) and fourier transform infrared (FTIR) analyses and then used in the formulation of herbal antacid tablets.

Results

The combination ratio of 3:1 (D. triflorum: P. heyneanus) demonstrated a significant activity (P < 0.05) in acid-neutralising effect assay (4.94 ± 0.00 end pH) and acid neutralisation duration assay (403.4 ± 4.6 seconds). The 3:1 combination further resulted in synergistic antacid activity, evidenced by the combinative index analysis. Membrane dialysed high- and low-molecular fractions of the same extract demonstrated bioactivities comparable to each other (P > 0.05) in antacid duration assay (182.78 ± 3.66 seconds and 218.18 ± 2.34 seconds respectively). The herbal tablet formulated using the 3:1 combination of the test plants showed pharmaceutically acceptable physical characteristics along with a significant acid-neutralisation (P < 0.05).

Conclusions

The 3:1 concentration combination of D. triflorum and P. heyneanus extracts resulted in synergistic gastric acid-neutralising effects, possibly with the aid of phytochemicals with various molecular weights. The combination can be further used in an antacid tablet formulation with sufficient in vitro activity.

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来源期刊

Journal of Herbal Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.