Porometer for estimating stomatal conductance in maize: Determination of trueness and precision according to ISO 5725

Abstract

Stomatal conductance (g_s) is a key variable for quantifying crop water status and different technologies have been developed for its determination. While infrared gas analysers (IRGA) are widely recognised as a reference for g_s measurement, their limited usability and portability, and their cost, are making porometers an increasingly seductive alternative. However, few studies have compared porometers with other methods, and key information on performance metrics along the g_s biophysical range is missing. The accuracy (precision and trueness) of the LI-600 porometer for g_s measurement in maize was evaluated using the ISO 5725 protocol. A ring trial was carried out by growing plants in pots under three irrigation regimes to identify different g_s levels. Measurements of g_s were performed by three independent groups of operators at two growth stages using the porometer and the IRGA (reference). For intermediate g_s values, precision was satisfactory (mean relative standard deviation of repeatability and reproducibility <19%), whereas marked underestimations were observed. In cases of severely stressed and well-watered plants, the trueness was good (overall R² was 0.62), whereas the poor precision could be compensated by the possibility of taking a high number of replicates (very short time is needed for acquiring data). This, together with the high usability, make porometers an alternative in the case of intensive or time-constrained field campaigns.