Genetic and haplotype diversity of redbreast tilapia (Coptodon rendalli) based on cytochrome oxidase subunit I and D-loop

IF 3.7 3区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Mehrnoush Aminisarteshnizi, Ngonidzashe A.G. Moyo, Mokgadi E. Raphalo
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Abstract

The genetic diversity in Redbreast tilapia (Coptodon rendalli) is under threat from climate change, human activities, and natural hybridisation, prompting conservation concerns. To investigate the genetic characteristics of C. rendalli, genetic diversity, genetic structure, population differentiation, and historical demography were evaluated using mitochondrial DNA COI (cytochrome oxidase subunit I) and D-loop sequences from various countries. Phylogenetic and genetic diversity analysis based on the mtDNA sequences (COI H = 9, haplogroup = 3, and D-loop H = 5, haplogroup = 3) showed private haplotypes in some of the populations. The results showed significant differences in genetic diversity among the analysed populations (P < 0.001). Molecular variance analysis showed low variation levels among populations (COIFST = 0.02176, D-loopFST = 0.52), which was significant only for D-loop. The neutrality tests and mismatch distribution based on the mtDNA supported the hypothesis of under-purifying selection of C. rendalli. The results indicated that the population did not undergo expansion. The findings of this study revealed molecular divergence among the C. rendalli populations and may serve as pivot information for the genetic improvement of this species. In conclusion, this study has elucidated the utility of two reliable and cost-effective molecular markers for analysing genetic diversity. Notably, the mitochondrial D-loop region’s sensitivity to reflecting genetic diversity in C. rendalli surpasses that of the COI gene.
基于细胞色素氧化酶I亚基和d环的红胸罗非鱼遗传多样性和单倍型多样性
红胸罗非鱼(Coptodon rendalli)的遗传多样性受到气候变化、人类活动和自然杂交的威胁,引起了人们对其保护的关注。为了研究黄颡鱼的遗传特征,利用来自不同国家的线粒体DNA COI(细胞色素氧化酶亚基I)和d环序列,对黄颡鱼的遗传多样性、遗传结构、种群分化和历史人口统计学进行了评估。基于mtDNA序列(COI H = 9, haplogroup = 3, D-loop H = 5, haplogroup = 3)的系统发育和遗传多样性分析显示,部分群体存在私有单倍型。结果显示,所分析群体间遗传多样性存在显著差异(P <;0.001)。分子方差分析显示群体间COIFST = 0.02176, D-loopFST = 0.52,差异不显著,仅D-loop差异显著。基于mtDNA的中性性检验和错配分布支持了青霉的低净化选择假说。结果表明,种群未发生扩张。本研究的结果揭示了青檀居群间的分子分化,可作为青檀遗传改良的支点信息。总之,本研究阐明了两种可靠且经济的分子标记在遗传多样性分析中的应用。值得注意的是,C. rendalli线粒体D-loop区域对反映遗传多样性的敏感性超过了COI基因。
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来源期刊
Journal of King Saud University - Science
Journal of King Saud University - Science Multidisciplinary-Multidisciplinary
CiteScore
7.20
自引率
2.60%
发文量
642
审稿时长
49 days
期刊介绍: Journal of King Saud University – Science is an official refereed publication of King Saud University and the publishing services is provided by Elsevier. It publishes peer-reviewed research articles in the fields of physics, astronomy, mathematics, statistics, chemistry, biochemistry, earth sciences, life and environmental sciences on the basis of scientific originality and interdisciplinary interest. It is devoted primarily to research papers but short communications, reviews and book reviews are also included. The editorial board and associated editors, composed of prominent scientists from around the world, are representative of the disciplines covered by the journal.
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