Therapeutic potential of Chemogenic Iron oxide Nanoparticles, its antioxidant and anti-Inflammatory effects with reduction of ankle joint swelling in BALB/c mice

Abstract

Background: Gout is a form of inflammatory arthritis caused by excessive deposition of uric acid in the body and joints. Prolonged administration of uric acid lowering drugs have multiple side effects. Objectives: To investigate the antioxidant and anti-gout properties of iron oxide nanoparticles in hyperuricemic gouty mice. Methodology: Sol-Gel precipitation technique was used for chemogenesis of Iron oxide nanoparticles by using Ferrous Chloride (FeCl2) and Ferric chloride (FeCl3). Characterization was carried out by X-ray diffraction, SQUID magnetization, scanning electron microscopy(SEM) and energy-dispersive X-ray spectroscopy(EDX). Monosodium Urate crystals (MSUC) induced gout into the ankle joint of the mice. Iron oxide nanoparticles were orally administrated to the mice. To study the impact of Iron oxide nanoparticles biochemical analysis like total protein, antioxidant activity, and ankle diameter were conducted. Results: Results showed that oral administration of iron oxide nanoparticles had a beneficiary impact on gouty mice. The antioxidant activity were significantly improved by increasing levels of thiobarbituric acid reactive substance and reactive oxygen species in treated group with 250 mg/kg of body weight of iron oxide nanoparticles. Ankle diameter and levels of total protein were also reduced in treated groups. Conclusion: Current research indicates that Iron oxide nanoparticles have antioxidant as well as anti-gout effects in gouty mice. These results provide credence to the possibility of using Iron oxide nanoparticles as a gout alternative treatment. The potential therapeutic uses of iron oxide nanoparticles in human patients require more investigation.