Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic dissemination

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano Pub Date : 2025-01-15 DOI:10.1016/j.onano.2025.100233

Sara Micheli , Caterina Piunti , Elisa Varaschin , Marianna Peditto , Maria Luz Suarez , Marco Sorgato , Elisa Cimetta

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引用次数: 0

Abstract

Detailed studies of cells migration are key in understanding tumors metastatic spread. We used two-photon polymerization (2PP) to create precise microdevices for studying cell migration through micro-channels at a single cell resolution. Micro-channels are designed to mimic the structure of lymphatic vessels, conduits for cell movement in vivo. Neuroblastoma (NB) and human Mesenchymal Stem Cells (MSCs) represent the main tumor and its primary metastatic site. Our results revealed distinctive behaviors of NB and MSCs, both individually and in co-culture, hinting at a tumor-suppressive role of MSCs inhibiting NB migration. Pre-exposure of MSCs to NB-derived extracellular vesicles (EVs) significantly increased their motility towards tumor cells. Our platform more effectively replicates the in vivo environment of metastatic migration, with results providing new insights into the early dissemination of NB. Such microdevices hold great promise for advancing our understanding of metastasis and aiding the development of targeted anti-cancer therapies.

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双光子光刻微通道阵列装置在神经母细胞瘤转移传播中的细胞迁移研究

细胞迁移的详细研究是了解肿瘤转移扩散的关键。我们使用双光子聚合（2PP）来制造精确的微设备，用于在单细胞分辨率下研究细胞通过微通道的迁移。微通道是为了模拟淋巴管的结构而设计的，淋巴管是细胞在体内运动的管道。神经母细胞瘤（NB）和人间充质干细胞（MSCs）是主要的肿瘤及其原发性转移部位。我们的研究结果揭示了NB和MSCs在单独和共培养中的独特行为，暗示MSCs抑制NB迁移的肿瘤抑制作用。将MSCs预先暴露于nb来源的细胞外囊泡（ev）可显著增加其对肿瘤细胞的运动性。我们的平台更有效地复制了转移性迁移的体内环境，其结果为NB的早期传播提供了新的见解。这样的微型设备在促进我们对转移的理解和帮助靶向抗癌治疗的发展方面有着巨大的希望。

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来源期刊

OpenNano Medicine-Pharmacology (medical)

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

50 days

期刊介绍： OpenNano is an internationally peer-reviewed and open access journal publishing high-quality review articles and original research papers on the burgeoning area of nanopharmaceutics and nanosized delivery systems for drugs, genes, and imaging agents. The Journal publishes basic, translational and clinical research as well as methodological papers and aims to bring together chemists, biochemists, cell biologists, material scientists, pharmaceutical scientists, pharmacologists, clinicians and all others working in this exciting and challenging area.