Comparative analysis of the chloroplast genomes of different strains of Pyropia haitanensis

IF 1.9 4区生物学 Q2 MARINE & FRESHWATER BIOLOGY

Aquatic Botany Pub Date : 2024-12-12 DOI:10.1016/j.aquabot.2024.103854

Hongzi Song , Zhicong Gao , Haimin Chen , Shanshan Zhu

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引用次数: 0

Abstract

Pyropia haitanensis, an economically and nutritionally significant marine crop, is extensively cultivated in China. This study employed genome skimming to assemble and annotate the chloroplast genome sequences of 15 different strains of P. haitanensis and conducted a comparative analysis of the chloroplast genome structure and characteristics. The assembled chloroplast genomes ranged in size from 195,577 to 201,312 base pairs (bp) and contained annotations for 253 (or 254 in some strains) genes associated with photosynthesis, transcription, biosynthesis and other essential processes. The number of simple repeat sequences (SSRs) varied between 28 and 30, with AT as the most abundant two-base repeat sequence. Despite overall conservation in the chloroplast genome structure, we identified eight highly variable regions that can serve as potential molecular markers for distinguishing different strains of P. haitanensis. These regions are eight intergenic spacer regions: petJ-carA, ORF32-ycf35, rbl35-pbsA, ORF68-accB, psbB-psbT, syh-groEL, ORF107-ycf12, rpl32-ORF263. Phylogenetic analysis indicates that the 15 strains mainly divide into three subgroups, suggesting a degree of genetic differentiation among them. Overall, this study not only contributed to the understanding of the genetic variation of P. haitanensis, but also offered valuable resources for future research, breeding efforts, and utilization of this economically important marine crop.

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来源期刊

Aquatic Botany 生物-海洋与淡水生物学

CiteScore

3.80

自引率

5.60%

发文量

审稿时长

6 months

期刊介绍： Aquatic Botany offers a platform for papers relevant to a broad international readership on fundamental and applied aspects of marine and freshwater macroscopic plants in a context of ecology or environmental biology. This includes molecular, biochemical and physiological aspects of macroscopic aquatic plants as well as the classification, structure, function, dynamics and ecological interactions in plant-dominated aquatic communities and ecosystems. It is an outlet for papers dealing with research on the consequences of disturbance and stressors (e.g. environmental fluctuations and climate change, pollution, grazing and pathogens), use and management of aquatic plants (plant production and decomposition, commercial harvest, plant control) and the conservation of aquatic plant communities (breeding, transplantation and restoration). Specialized publications on certain rare taxa or papers on aquatic macroscopic plants from under-represented regions in the world can also find their place, subject to editor evaluation. Studies on fungi or microalgae will remain outside the scope of Aquatic Botany.