Use of a microvascular anastomotic coupler device for kidney transplantation in rats

IF 1.4 Q3 SURGERY
Henrik Lauer, Jana Ritter, Patrick Nachtnebel, Kathrin Simmendinger, Emily Lerchbaumer, Vladyslav Kavaka, Dominik Steiner, Jonas Kolbenschlag, Adrien Daigeler, Johannes C. Heinzel
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Abstract

Background

Allogenic kidney transplantation has been the gold standard treatment for end-stage renal disease. In the research setting, rat models are widely utilized to refine surgical techniques and enhance graft viability. One critical factor affecting transplantation outcomes is the efficiency of the venous anastomosis. This study evaluates the utility of a microvascular coupling device for venous anastomosis in a rat kidney transplantation model.

Material and methods

Experimental allogenic kidney transplantations were conducted in male Brown Norway rats (n = 10) as donors and Lewis rats as recipients (n = 17), housed according to institutional guidelines. A microvascular coupling device was used for renal venous anastomosis, and creatinine levels were measured postoperatively to assess kidney function. Procedure times, ischemia duration, and postoperative complications were recorded and analyzed.

Results

The venous anastomosis time averaged 6.6 ± 2.2 min. Total ischemia time averaged 42.4 ± 4.9 min. Early postoperative serum creatinine levels were slightly elevated about references thresholds, which normalized by postoperative day 3. Four animals died after successful transplantation due to urethral complications and postrenal failure (23.5 %). Other postoperative mortality was primarily linked to complications unrelated to thrombosis (n = 3, 17.6 %).

Conclusion

The use of a microvascular coupling device for venous anastomosis in rat kidney transplantation significantly reduces procedure time and ischemia duration, contributing to more consistent graft outcomes. The simplification of the venous anastomosis process and reduced operative time justify the use of coupling devices. This technique holds promise for advancing preclinical transplant research and improving reproducibility in microsurgical procedures.
微血管吻合耦合器在大鼠肾移植中的应用
背景:同种异体肾移植一直是治疗终末期肾脏疾病的金标准。在研究环境中,大鼠模型被广泛用于改进手术技术和提高移植物活力。影响移植结果的一个关键因素是静脉吻合的效率。本研究评估微血管耦合装置在大鼠肾移植模型静脉吻合中的应用。材料与方法实验采用雄性布朗挪威大鼠(n = 10)作为供体,Lewis大鼠(n = 17)作为受体,按照机构指南饲养。采用微血管耦合装置进行肾静脉吻合,术后测定肌酐水平评价肾功能。记录并分析手术时间、缺血持续时间和术后并发症。结果静脉吻合时间平均6.6±2.2 min,总缺血时间平均42.4±4.9 min,术后早期血清肌酐水平略高于参考阈值,术后第3天恢复正常。移植成功后因尿道并发症和肾后衰竭死亡4只(23.5%)。其他术后死亡率主要与与血栓无关的并发症有关(n = 3, 17.6%)。结论在大鼠肾移植中应用微血管耦合装置进行静脉吻合可显著缩短手术时间和缺血时间,使移植结果更加一致。静脉吻合过程的简化和手术时间的缩短证明了耦合装置的使用是合理的。这项技术有望推进临床前移植研究,提高显微外科手术的可重复性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.30
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