In-vitro microbicidal activity of Ganoderma lucidum aqueous extract against selected pathogenic bacteria and yeast

Abstract

Introduction

The potential antibacterial properties of aqueous extracts of Ganoderma lucidum, particularly its ability to combat pathogenic bacteria and fungi, have captivated the interest of both the scholarly and pharmaceutical sectors. This investigation was conducted to explore the antimicrobial efficacy of aqueous extracts of G. lucidum: Sample A (subjected to boiling for 1 hour) and Sample B (subjected to boiling for 3 hours) against pathogenic bacteria and yeast.

Method

Standard microbiological procedures were used to isolate, identify, and determine the antimicrobial properties of the extracts. A standard procedure was utilised to prepare the samples.

Results

As evidenced in the study, a notable disparity was observed in the impact of the Sample A extract on the test isolates, with the most pronounced effect noted on Staphylococcus spp at a concentration of 1 000 mg/ml (10.33 ± 0.88 mm). Conversely, no antimicrobial activity (0.00 ± 0.00 mm) was detected in any concentration against E. coli, Enterobacter spp, Bacillus spp, and Candida spp. Moreover, the findings pertaining to Sample B indicated that the most substantial effect was observed on Pseudomonas spp at 1 000 mg/ml (6.67 ± 0.33 mm). Conversely, no antimicrobial efficacy (0.00 ± 0.00 mm) was observed in all concentrations of the extract against Staphylococcus spp and Candida spp, while E. coli, Enterobacter spp, and Bacillus spp exhibited no activity at concentrations of 125 and 62.5 mg/ml.

Conclusion

These findings demonstrate the presence of bioactive substances with a wide range of antibacterial action in G. lucidum confirming assertions of the traditional herbalists that some bacterial diseases in people may be treated with Ganoderma species, hence the need for further study on compound isolation guided bio-assay.