MALAT1 and BCYRN1 lncRNAs expression in patients with breast cancer from Northwest Iran

IF 0.5 Q4 GENETICS & HEREDITY

Human Gene Pub Date : 2025-02-01 DOI:10.1016/j.humgen.2024.201370

Alireza Ahmadi, Amin Moqadami, Mohammad Khalaj-Kondori, Mohammad Ali Hosseinpour Feizi

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Abstract

Introduction

Abnormally expressed lncRNAs in cancer tissues can potentially play as biomarkers for cancer diagnosis, prognosis, and treatment. This study aimed to assess the expression and biomarker potential of MALAT1 and BCYRN1 in breast cancer (BC).

Methods

Seventy paired samples of cancerous and non-cancerous adjacent tissues from breast cancer patients were collected and used for RNA extraction and cDNA synthesis. The expressions of MALAT1 and BCYRN1 lncRNAs were evaluated by qRT-PCR. In addition, the correlation between the expression levels of MALAT1 and BCYRN1 was investigated.

Results

The qRT-PCR results revealed that MALAT1 and BCYRN1 levels were considerably elevated in tumor tissues compared to the marginal samples of BC patients. Furthermore, MALAT1 expression was extremely correlated with tumor stages (p = 0.031). ROC curve analysis indicated that BCYRN1 might be considered a potential biomarker for BC, while MALAT1 showed poor results in this study. In addition, there was a statistically non-significant positive correlation between MALAT1 and BCYRN1 expression levels.

Conclusion

In conclusion, the expression levels of MALAT1 and BCYRN1 lncRNAs were significantly upregulated in breast cancer tissues compared to normal margins.

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