Vitamin D stimulates Il-15 synthesis in rodent muscle

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-01-25 DOI:10.1016/j.bbrep.2025.101925

Franz Ewendt , Fabienne Drewitz , Michael Althammer , Cosima Eichler , Corinna Brandsch , Stefanie Brey , Thomas H. Winkler , Mirja R. Wilkens , René St-Arnaud , Marina Kreutz , Gabriele I. Stangl

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引用次数: 0

Abstract

Besides its classical skeletal function, vitamin D plays a critical role in both skeletal muscle and the immune system. Interleukin-15 (IL-15), which is highly expressed, and secreted complexed with its receptor, IL-15Rα, by skeletal muscle, stimulates the development of immune cells and affects myogenesis and muscle mass. However, little is known about possible regulators of this myokine. To test whether vitamin D could be a regulator of muscle IL-15 and IL-15Rα expression, C2C12 myotubes were treated with vitamin D₃ metabolites and analysis were performed in gastrocnemius muscles of rats treated with a single intraperitoneal dose of 1,25(OH)₂D₃. The role of VDR was investigated by siRNA technique in C2C12 myotubes and in gastrocnemius muscles of vitamin D receptor knockout (Vdr-KO) mice. Treatment of C2C12 myotubes with 1,25(OH)₂D₃ or 25(OH)D₃ increased Il-15 gene expression in a dose-dependent manner and 1,25(OH)₂D₃ also moderately increased the relative Il-15 protein amount. Rats treated with a single dose of 1,25(OH)₂D₃ demonstrated a higher mRNA abundance of muscle Il-15 than controls. The 1,25(OH)₂D₃ effect on Il-15 was considerably weaker in C2C12 myotubes treated with Vdr-specific siRNA. Vdr-KO mice showed significantly lower muscle Il-15 mRNA than WT mice. Il-15Ra mRNA and Il-15/Il-15Rα protein abundance were unaffected by 1,25(OH)₂D₃-treatment or VDR functionality, and Cyp27b1 activity is not required for 25(OH)D₃-mediated Il-15 gene expression.

The results provide evidence for a regulatory role of hydroxyvitamin D₃ metabolites on the Il-15 synthesis in skeletal muscle cells, which is largely mediated by the VDR.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.