Ganoderma lucidum polysaccharide attenuates retinal ischemia-reperfusion injury by regulating microglial M1/M2 polarization, suppressing neuroinflammation and inhibiting JAK2/STAT3 pathway

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-01-29 DOI:10.1016/j.bbrep.2025.101926

Guangyu Zhu , Yujie Liu , Shichun Luo , Chao Tang , Chunlin Zhao , Xuejing Lu

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Abstract

Retinal ischemia-reperfusion (RIR) injury is implicated in the pathogenesis of numerous retinal degenerative disorders, resulting in visual impairment or even blindness in millions of individuals worldwide. In recent years, targeting the suppression of microglia-mediated neuroinflammation has emerged as a principal therapeutic strategy for RIR. Ganoderma lucidum polysaccharide (GLP), a pivotal bioactive extract of Ganoderma lucidum, has been demonstrated to possess efficacious anti-neuroinflammatory properties, while the precise impact of it on RIR injury remains incompletely elucidated. In this study, the RIR model was induced in Sprague-Dawley rats by elevating the intraocular pressure to 80 mmHg for 60 min. Our findings revealed that GLP significantly alleviated inflammatory processes by impeding the secretion of pro-inflammatory cytokines while facilitating that of anti-inflammatory cytokines. Moreover, the administration of GLP also promoted the polarization of microglia from the M1 phenotype to the M2 phenotype. Further investigation of the treatment mechanism indicated that the regulatory effects of GLP were presumably mediated by the inhibition of the JAK2/STAT3 signaling pathway. To summarize, we provided a novel insight into the mechanisms by which GLP ameliorated RIR injury, thereby indicating that it could be identified as a promising candidate for the management of RIR-related diseases.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.