Chitosan and carbon nanotube modified DNA biosensor for determination of drug-DNA interaction

Abstract

In the present study Chitosan (CHIT) and Multiwalled carbon nanotube (MWCNT) modified pencil graphite electrodes (MWCNT-CHIT-PGEs) were developed for enhanced electrochemical monitoring of nucleic acid, and the interaction of the anticancer drug Mitomycin C (MC) and calf thymus double stranded DNA (ctdsDNA). The microscopic characterization of PGE and MWCNT-CHIT-PGE surfaces was carried out by scanning electron microscopy (SEM). Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) techniques were used to evaluate the electrochemical performance of the MWCNT-CHIT-PGEs. The interaction between MC and ctdsDNA was investigated by measuring the oxidation signals of both MC and guanine in the same voltammetric scale using Differential pulse voltammetry (DPV) technique. The experimental parameters, dsDNA and MC concentration and the interaction time were optimized to enhance the overall efficiency of the experimental process.