Fluorescent tetrazolylpyrene unnatural nucleoside in sensing BSA protein

Abstract

A spectroscopic investigation of the interaction of a fluorescent unnatural tetrazolylpyrene nucleoside (^TPyB_Do) with model protein BSA is presented herein. The probe nucleoside shows a good interaction with BSA, which is reflected by the enhanced fluorescence intensity with an increase in the concentration of BSA. The enhancement is found to be linear with respect to the BSA concentration. Thus, the probe might find applications in chemical biology. The results infer that exploiting weak non-covalent interactions, such as H-bonding/hydrophobic, is an efficient strategy for sensing biomolecules. Further, the result would also help in designing other novel fluorescent probes capable of interacting with biomolecules via such forces with the generation of enhanced signals.