Cost effective optimization of bacteria removal in Nannochloropsis oceanica concentrates

IF 3.6 2区农林科学 Q2 AGRICULTURAL ENGINEERING

Aquacultural Engineering Pub Date : 2025-01-20 DOI:10.1016/j.aquaeng.2025.102513

Cynthia Couto , Fabio Roselet , Bruno Kubelka , Fernando Gonçalves , Robson Matheus Marreiro Gomes , Juan Rafael Buitrago Ramírez , José Maria Monserrat , Dariano Krummenauer

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Abstract

The objective of this study was to decrease bacterial contamination and improve the net benefit-cost of the process while preserving the integrity of microalgae cells. The washing process conditions were optimized to increase the rate of bacterial removal, making the process economically viable without impacting cell viability. To this, a Central Composite Rotational Design with four variables and two levels (CCRD 2⁴) was performed, generating 27 experimental units (runs). Cell viability was determined by subculture in culture medium and growth rate calculation. The rate of bacterial removal was estimated by directly counting bacteria in the concentrate before and after undergoing the washing process, and a Partial Budget Analysis estimated the net benefit-cost for each run. The experimental models of each response were combined using the desirability index to find the combination that generated the best result in all responses. The best relation between cell viability, bacterial removal efficiency rate, and PBA was found by repeating the process 5 times at 3.405 g-force at 24 °C for 20 min. To make the washing process economically viable, it was necessary to increase the sales price by 10 %, which is justified by the increase in shelf life that the process generates.

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海洋纳米绿藻浓缩液除菌的成本效益优化

本研究的目的是减少细菌污染，提高该过程的净效益-成本，同时保持微藻细胞的完整性。优化了洗涤工艺条件，以提高细菌去除率，使该工艺在不影响细胞活力的情况下经济可行。为此，进行了四变量两水平的中心复合旋转设计（CCRD 24），产生了27个实验单元（运行）。通过培养基传代和计算细胞生长速率来测定细胞活力。通过在洗涤过程前后直接计数浓缩物中的细菌来估计细菌去除率，并进行部分预算分析来估计每次运行的净效益-成本。利用期望指数将每个反应的实验模型组合起来，以找到在所有反应中产生最佳结果的组合。在3.405 g, 24 °C， 20 min的条件下重复5次，发现细胞活力、细菌去除率和PBA之间的最佳关系。为了使洗涤过程在经济上可行，有必要将销售价格提高10% %，这是合理的，因为该过程产生的保质期增加了。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquacultural Engineering 农林科学-农业工程

CiteScore

8.60

自引率

10.00%

发文量

审稿时长

>24 weeks

期刊介绍： Aquacultural Engineering is concerned with the design and development of effective aquacultural systems for marine and freshwater facilities. The journal aims to apply the knowledge gained from basic research which potentially can be translated into commercial operations. Problems of scale-up and application of research data involve many parameters, both physical and biological, making it difficult to anticipate the interaction between the unit processes and the cultured animals. Aquacultural Engineering aims to develop this bioengineering interface for aquaculture and welcomes contributions in the following areas: – Engineering and design of aquaculture facilities – Engineering-based research studies – Construction experience and techniques – In-service experience, commissioning, operation – Materials selection and their uses – Quantification of biological data and constraints