Deep-eutectic solvent extraction of ginsenosides and ginseng polysaccharides from ginseng residue and utilization of post-extraction residue

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry Pub Date : 2025-02-01 DOI:10.1016/j.procbio.2024.12.003

Zhaofeng Guan , Lina Zhou , Hao Wu , Zhenyu Chu , Mingjun Liu , Jun Zhou

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引用次数: 0

Abstract

In this study, ginsenosides and polysaccharides were extracted from ginseng residue (GR) using a green method combining deep eutectic solvent (DES) and ultrasound. The results showed that the optimal extraction conditions of ginsenosides optimized by orthogonal test were choline chloride/propionic acid ratio of 1:3, moisture content of 30 %, solid-liquid ratio of 1:25, and ultrasound time of 30 min, which resulted in the yield of ginsenosides of 36.76 ± 1.46 mg/g, and improved the efficiency by 218.27 % compared with that of the traditional 70 % ethanol extraction method. The ginseng polysaccharides obtained by further purification of the extract showed different in vitro antioxidant activities, with scavenging rates of 91.2 %, 98.3 %, and 96.4 % for hydroxyl radicals, DPPH, and superoxide radicals, respectively, and Fe(Ⅱ) chelating activity at 93.5 %. The extracted GR showed good adsorption properties for glucose, sodium nitrite, cholesterol, and sodium cholate by in vitro adsorption tests with adsorption rates of 60 %, 93.3 %, 87.05 %, and 72.95 %, respectively. In conclusion, the use of DES as an extraction solvent has certain application prospects for GR reuse.

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来源期刊

Process Biochemistry 生物-工程：化工

CiteScore

8.30

自引率

4.50%

发文量

374

审稿时长

53 days

期刊介绍： Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.