Effect of Cinnamomum cassia essential oil combined with enzymes on the elimination and regrowth potential of Listeria monocytogenes and Salmonella enterica biofilms formed on stainless steel surfaces

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control Pub Date : 2025-01-20 DOI:10.1016/j.foodcont.2024.111120

Cervantes-Huamán B.R.H., Vega-Sánchez A., Rolón-Verdún P., Gervilla-Cantero G., Rodríguez-Jerez J.J., Ripolles-Avila C

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引用次数: 0

Abstract

This study investigates the biofilm-forming potential of five distinct Listeria monocytogenes (ST-388, S2-bac, EDG-e, CECT 5672, and CECT 935) and Salmonella enterica strains (CECT 4594, CECT 4156, CECT 4300, CECT 4155, and CECT 4396), and evaluates the efficacy of various treatments involving Cinnamomum cassia essential oil (EO) and/or the enzymes protease, amylase, and mannanase on biofilms formed on stainless steel surfaces. Employing both quantitative and qualitative methodologies (i.e., TEMPO system and Direct Epifluorescence Microscopy (DEM)), the research aims to elucidate the biofilm formation dynamics and treatment outcomes. No significant differences were observed (P < 0.05) among the eight treatments applied to the model strain ST-388, while for S. Typhimurium treatments from A1 to A4 were the most effective (P > 0.05). Quantitative and qualitative evaluations showed significant biofilm cell detachment, with reductions ranging from 4.97 ± 0.85 to 6.19 ± 0.56 Log CFU/cm², depending on the strain and treatment applied. The antimicrobial activity of C. cassia EO, primarily due to trans-cinnamaldehyde, was effective against both pathogens and increased its inhibition when applied with the enzymatic formulation. Imaging analyses confirmed the structural breakage of biofilms, highlighting strain-specific responses to the treatments. Although none of the treatments achieved complete eradication of biofilms, the remaining cell load varied among strains, influencing the regeneration potential. Surviving cells were capable of consolidating new biofilm structures during subsequent incubation. However, regrowth levels did not fully reach the initial biofilm density. These findings underscore the potential of alternative approaches utilizing natural compounds and enzymatic treatments to enhance biofilm control strategies within the food industry's standard cleaning and disinfection protocols.

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来源期刊

Food Control 工程技术-食品科技

CiteScore

12.20

自引率

6.70%

发文量

758

审稿时长

33 days

期刊介绍： Food Control is an international journal that provides essential information for those involved in food safety and process control. Food Control covers the below areas that relate to food process control or to food safety of human foods: • Microbial food safety and antimicrobial systems • Mycotoxins • Hazard analysis, HACCP and food safety objectives • Risk assessment, including microbial and chemical hazards • Quality assurance • Good manufacturing practices • Food process systems design and control • Food Packaging technology and materials in contact with foods • Rapid methods of analysis and detection, including sensor technology • Codes of practice, legislation and international harmonization • Consumer issues • Education, training and research needs. The scope of Food Control is comprehensive and includes original research papers, authoritative reviews, short communications, comment articles that report on new developments in food control, and position papers.