Biocontrol of gray spot disease on Polygonatum cyrtonema Hua by Bacillus velezensis GX1

IF 3.7 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control Pub Date : 2025-02-01 DOI:10.1016/j.biocontrol.2025.105704

L. Sun , G.L. Deng , H.L. Wu , Q. He , S.H. Hu , J.Y. Li , X.Y. Li , S. Meng , A.N. He , Y.Q. Tian , X. Chen , S.H. Li

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引用次数: 0

Abstract

This study evaluated the biocontrol efficacy of Bacillus velezensis GX1 against gray spot disease (Pestalotiopsis xuefengensis) on the horticultural species, Polygonatum cyrtonema Hua. Results indicated that GX1 effectively inhibited P. xuefengensis HJHB1, inhibiting growth of the pathogen by 74.97 % in vitro and inhibiting disease development by 87.13 % in field experiments. Notably, a 10 % (v/v) concentration of cell-free supernatant (CFS) of GX1 fully inhibited mycelial growth. An extract of lipopeptides (LPs) prepared at 60 μg/ml strongly inhibited mycelial growth and spore germination and caused morphological deformation of the mycelia. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis revealed that strain GX1 produced significantly higher levels of surfactin A, surfactin B and plipastatin, relative to strain GX8, an ineffective strain. Commercial preparations of plipastatin and surfactin indicated that plipastatin exhibited strong antifungal activity but surfactin did not. A bacterial solution of GX1 had no significant effect on superoxide dismutase (SOD) activity in P. cyrtonema but did enhance the activity of peroxidase (POD) and decreased catalase (CAT) activity, which are associated with increased resistance of P. cyrtonema to gray spot disease.

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来源期刊

Biological Control 生物-昆虫学

CiteScore

7.40

自引率

7.10%

发文量

220

审稿时长

63 days

期刊介绍： Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. The aim of Biological Control is to promote this science and technology through publication of original research articles and reviews of research and theory. The journal devotes a section to reports on biotechnologies dealing with the elucidation and use of genes or gene products for the enhancement of biological control agents. The journal encompasses biological control of viral, microbial, nematode, insect, mite, weed, and vertebrate pests in agriculture, aquatic, forest, natural resource, stored product, and urban environments. Biological control of arthropod pests of human and domestic animals is also included. Ecological, molecular, and biotechnological approaches to the understanding of biological control are welcome.