A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae

IF 3.7 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control Pub Date : 2025-02-01 DOI:10.1016/j.biocontrol.2024.105686

Pavan Kumar , Giandomenico Corrado , Girigowda Manjunatha , Suma Ramegowda , Pradeep Kumar , Boris Basile , Ganadalu Puttaswamy Mutthuraju , Baggana Girish Jasmitha

{"title":"A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae","authors":"Pavan Kumar , Giandomenico Corrado , Girigowda Manjunatha , Suma Ramegowda , Pradeep Kumar , Boris Basile , Ganadalu Puttaswamy Mutthuraju , Baggana Girish Jasmitha","doi":"10.1016/j.biocontrol.2024.105686","DOIUrl":null,"url":null,"abstract":"<div><div>Plant growth-promoting bacteria (PGPB) have emerged as sustainable tool for managing plant diseases. This study investigates the potential of a <em>Pseudomonas</em>-based biocontrol agent to manage bacterial blight (BB) in pomegranate. This major disease is caused by <em>Xanthomonas axonopodis</em> pv. <em>punicae</em> (Xap) and it is traditionally controlled with antibiotics. Of the 151 bacterial isolates obtained from the pomegranate rhizosphere, three (UHSPS15A, UHSPS33, and UHSPS54) demonstrated the strongest inhibitory effects against Xap <em>in vitro</em>, and their identification as <em>Pseudomonas</em> was confirmed through DNA analysis. Greenhouse trials with Xap-inoculated plants revealed that preventive application of each of the three isolates was more effective than curative, with UHSPS15A providing the highest protection. A talc-based formulation was developed using UHSPS15A. After evaluating its stability and efficacy in greenhouse Xap-inoculated pomegranates plants, open-fields trials indicated that among the three different treatment modes tested, the combined soil and foliar application achieved the highest disease protection and fruit yield, topping the standard antibiotic control. These findings recommend that integrating <em>Pseudomonas</em>-based bio-formulations into disease management strategies could significantly reduce reliance on synthetic chemicals, offering a sustainable alternative for controlling BB in pomegranate.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"201 ","pages":"Article 105686"},"PeriodicalIF":3.7000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Control","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1049964424002512","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Plant growth-promoting bacteria (PGPB) have emerged as sustainable tool for managing plant diseases. This study investigates the potential of a Pseudomonas-based biocontrol agent to manage bacterial blight (BB) in pomegranate. This major disease is caused by Xanthomonas axonopodis pv. punicae (Xap) and it is traditionally controlled with antibiotics. Of the 151 bacterial isolates obtained from the pomegranate rhizosphere, three (UHSPS15A, UHSPS33, and UHSPS54) demonstrated the strongest inhibitory effects against Xap in vitro, and their identification as Pseudomonas was confirmed through DNA analysis. Greenhouse trials with Xap-inoculated plants revealed that preventive application of each of the three isolates was more effective than curative, with UHSPS15A providing the highest protection. A talc-based formulation was developed using UHSPS15A. After evaluating its stability and efficacy in greenhouse Xap-inoculated pomegranates plants, open-fields trials indicated that among the three different treatment modes tested, the combined soil and foliar application achieved the highest disease protection and fruit yield, topping the standard antibiotic control. These findings recommend that integrating Pseudomonas-based bio-formulations into disease management strategies could significantly reduce reliance on synthetic chemicals, offering a sustainable alternative for controlling BB in pomegranate.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Biological Control 生物-昆虫学

CiteScore

7.40

自引率

7.10%

发文量

220

审稿时长

63 days

期刊介绍： Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. The aim of Biological Control is to promote this science and technology through publication of original research articles and reviews of research and theory. The journal devotes a section to reports on biotechnologies dealing with the elucidation and use of genes or gene products for the enhancement of biological control agents. The journal encompasses biological control of viral, microbial, nematode, insect, mite, weed, and vertebrate pests in agriculture, aquatic, forest, natural resource, stored product, and urban environments. Biological control of arthropod pests of human and domestic animals is also included. Ecological, molecular, and biotechnological approaches to the understanding of biological control are welcome.