Lnc_CXADR/miR-421/ATG7 as promising biomarkers relevant to hepatocellular carcinoma

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2024-12-10 DOI:10.1016/j.genrep.2024.102114

Dalia Abdel Wahab Mohamed , Raghda M. Ghorab , Soha Abuelela , Dina Morsy , Wael M. Elayat

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引用次数: 0

Abstract

Noncoding RNAs (ncRNAs) are a diverse class of non-protein coding RNA molecules. They have gained significant attention as key regulators of gene expression, contributing to the complex molecular landscape of cancer. There has been a growing concern about ncRNAs' diagnostic and prognostic potential as biomarkers in hepatocellular carcinoma (HCC) and their therapeutic implications in HCC treatment. This study focuses on elucidating the role of microRNAs (miRNAs) and long noncoding RNAs (LncRNAs); being two of the major classes of ncRNAs that are suggested to play essential roles in the initiation and progression of HCC.

Methods

In silico analysis was used to retrieve biomarkers associated with HCC. We used public microarray databases to explore ncRNAs relevant to HCC; has-mir-421 and lncRNA-CXADR were chosen. Their relative expression and ATG7 as an autophagy marker were evaluated by Real-time quantitative PCR (RT-qPCR) in 3 patient groups: HCC, patients with hepatitis C virus (HCV), and a control group.

Results

Findings revealed that has-mir-421, Lnc_CXADR, and ATG7 expression in the HCC group was significantly elevated than in HCV and control groups (P < 0.01). Furthermore, a strong significant positive correlation existed between has-mir-421, Lnc_CXADR, and ATG7 expression (p < 0.01). This positive correlation indicates that Lnc_CXADR may upregulate the expression of miRNA421, triggering upregulation of ATG7 expression in HCC, which may have an oncogenic role in HCC pathogenesis.

Conclusion

Lnc_CXADR/miRNA421/ATG7 may play promising roles in HCC diagnosis and prognosis as new serum biomarkers.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.