ID1 gene overexpression confers quiescence and chemoresistance in a leukemia cellular model

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2024-12-08 DOI:10.1016/j.genrep.2024.102110

José L. Padilla Agudelo , Diego F. Rincón Reyes , Karen L. Pachón Meza , Andrea I. Pinilla Da Silva , Camilo Ernesto Moreno Cristancho , Nataly Cruz Rodriguez , José A. Gutiérrez Triana

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引用次数: 0

Abstract

Introduction

B-precursor acute lymphoblastic leukemia (B-ALL) is a hematological malignancy with a high incidence in Colombia and a low survival rate compared to other countries. Previous studies found a significant association between the altered expression of the ID1, ID3, and IGJ genes with poor disease prognosis in Colombian adult patients.

Method and main results: In order to understand the fundamental processes involved, we created a B-ALL cell line (NALM-6) that constitutively overexpress the ID1 gene, using the Tol2-transposase system.

Conclusions

The NALM6_tID1 cells consistently exhibited increased expression of ID1, leading to reduced cell growth and notable resistance to dexamethasone and cyclophosphamide chemotherapy drugs, when compared to the control group. This study provides important information for developing personalized treatment options by improving our understanding of the molecular pathways that cause B-ALL in patients from Colombia.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.