Influence of platelet-rich plasma on RANKL and IL-1 immunohistochemical expression in periodontitis-related bone cell proliferation and differentiation

IF 2.3 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Saudi Dental Journal Pub Date : 2024-12-01 DOI:10.1016/j.sdentj.2024.11.011

Hana H. Mustafa , Snur M.A. Hassan , Sozan Ali Mohammed , Mardin O. Mohammed , Hadia Karim Zorab , Hardi Fattah Marif

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引用次数: 0

Abstract

Background

Platelet-rich plasma (PRP) is utilized as an autologous blood product to encourage bone regeneration. The receptor activator of nuclear factor-NB ligand (RANKL) is a key and central regulator of osteoclast homeostasis. A rat model of experimentally generated periodontitis was used to assess the impact of PRP preparation on the expression of the osteoclastogenic and pro-inflammatory markers respectively; RANKL and IL-1β.

Material and Methods

To induce periodontitis by silk ligature, thirty-six adult male Sprague Dawleys rats were used and they were allocated into three equal groups (n = 12): group I consisted of intact periodontal tissue, group II; rat-induced periodontitis without treatment by PRP, and group III of periodontitis + 10 µL PRP injection. The rats were sacrificed after both experiment durations (7 and 30 days), and the incisor teeth were fixed and decalcified in HCl for a day and in 10 % EDTA solution for eight weeks at room temperature then samples were processed for H&E stain for bone healing scores and bone cells counting, and the samples were utilized by IHC for detection of both RANKL and IL-1β expression.

Results

The PRP enhanced the process of healing on days 7 and 30 showed (Score 10) vs. the control positive group that had a delay in alveolar bone regarded as (Score 4) significantly (P ≤ 0.05). The PRP group attenuated significantly (P ≤ 0.05) the alveolar bone loss by increasing the number of osteoblasts and declining the proliferation of osteoclast vs. the control positive group that revealed bone destruction due to rising osteoclast proliferation and decreasing the osteoblast proliferation significantly (P ≤ 0.05). PRP inhibited the IL-1β expression (score = 0) vs. the control positive group that showed moderate staining of positive cells detected in both inflammatory cells and endothelium (score = 4). Regarding the RANKL expression, the PRP reduced its expression in vs. the control positive group (score = 4 vs. 12 respectively).

Conclusion

PRP is an anabolic agent that enhances proliferation of osteoblast and inhibit the osteoclast differentiation by downregulation of IL-1β and RANKL.

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富血小板血浆对牙周炎相关骨细胞增殖和分化中RANKL和IL-1免疫组化表达的影响

富血小板血浆（PRP）被用作促进骨再生的自体血液制品。核因子- nb配体受体激活因子（RANKL）是破骨细胞稳态的关键中枢调节因子。采用实验性牙周炎大鼠模型，观察PRP制剂对破骨细胞和促炎标志物表达的影响；RANKL和IL-1β。材料与方法为采用丝扎法诱导牙周炎，选用成年雄性sd大鼠36只，随机分为3组（n = 12）：ⅰ组为完整牙周组织，ⅱ组为完整牙周组织；大鼠诱导的牙周炎未给予PRP治疗，第三组牙周炎+ PRP注射10µL。实验结束后（7和30 d）处死大鼠，固定门牙，HCl脱钙1天，10% EDTA溶液脱钙8周，室温下进行H&；E染色进行骨愈合评分和骨细胞计数，免疫组化检测RANKL和IL-1β表达。结果PRP在第7天和第30天明显促进牙槽骨愈合（评分10分），与对照组相比，牙槽骨延迟（评分4分）显著（P≤0.05）。与对照组相比，PRP组通过增加成骨细胞数量、降低破骨细胞增殖而显著减轻牙槽骨丢失（P≤0.05），而对照组则通过增加破骨细胞增殖、显著降低成骨细胞增殖而出现骨破坏（P≤0.05）。与对照组相比，PRP抑制IL-1β表达（评分= 0），对照组炎症细胞和内皮细胞均有中度阳性细胞染色（评分= 4）。在RANKL表达方面，PRP与对照组相比，PRP降低了其表达（评分分别为4和12）。结论prp是一种通过下调IL-1β和RANKL表达促进成骨细胞增殖、抑制破骨细胞分化的合成代谢剂。

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来源期刊

Saudi Dental Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

22 weeks

期刊介绍： Saudi Dental Journal is an English language, peer-reviewed scholarly publication in the area of dentistry. Saudi Dental Journal publishes original research and reviews on, but not limited to: • dental disease • clinical trials • dental equipment • new and experimental techniques • epidemiology and oral health • restorative dentistry • periodontology • endodontology • prosthodontics • paediatric dentistry • orthodontics and dental education Saudi Dental Journal is the official publication of the Saudi Dental Society and is published by King Saud University in collaboration with Elsevier and is edited by an international group of eminent researchers.