RNA Sequencing Data Analysis of Oligoasthenoteratozoospermia Patients' Sperms and Effects of Curcumin on the Expression of Some Genes during Sperm Cryopreservation and Freeze-Thawing Process.

IF 1.3 4区 医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Farnoosh Naseri, Elham Siasy, Seyed Abdolhamid Angaji, Maryam Peyvandi
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引用次数: 0

Abstract

Background: The cause of oligoasthenoteratozoospermia (OAT) is unclear. In this original study, we examined OAT patients' sperm using RNA-sequencing and studied the impact of curcumin on gene expression during sperm cryopreservation and freeze-thawing.

Methods: RNA-seq was performed using the Galaxy Europe server in IKHC (Imam Khomeini Hospital Complex), Tehran, Iran in 2023. Sperm samples were collected from 30 OAT patients and 30 healthy volunteers. Sperm parameters were analyzed, and samples were frozen with 20 μM curcumin at -196 °C for one week. Thawed samples were assessed for sperm parameters, and the expression levels of bax, bak, bcl-2, bclw, casp9, apaf-1, SOD, cat, and GPX4 genes were measured using RT-PCR.

Result: RNA-seq analysis showed increased expression of NANOS1, HSPA6, and ALOXE3 genes, while BHLHE41, Hey1, and PPM1D genes were down-regulated in OAT patients' sperm. Curcumin (20 μM) effectively preserves sperm parameters and motility during cryopreservation in healthy subjects and in OAT patients in particular. Cryopreserved sperm from both OAT patients and healthy individuals exhibited reduced expression of pro-apoptotic genes, increased expression of anti-apoptotic genes, and elevated levels of SOD and GPX4 genes.

Conclusion: Altered expressions of NANOS1, HSPA6, ALOXE3, BHLHE41, Hey1, and PPM1D genes likely contribute to OAT development. Additionally, curcumin protects sperm parameters during cryopreservation for both healthy individuals and OAT patients.

少弱异精子症患者精子RNA测序数据分析及姜黄素对精子冷冻和冻融过程中部分基因表达的影响
背景:寡弱性异卵精子症(OAT)的病因尚不清楚。在这项原始研究中,我们使用rna测序技术检测了OAT患者的精子,并研究了姜黄素对精子冷冻保存和冻融过程中基因表达的影响。方法:于2023年在伊朗德黑兰伊玛目霍梅尼医院(IKHC)使用Galaxy Europe服务器进行RNA-seq检测。从30名OAT患者和30名健康志愿者中收集精子样本。分析精子参数,用20 μM姜黄素-196℃冷冻一周。对解冻后的样品进行精子参数评估,并采用RT-PCR检测bax、bak、bcl-2、bclw、casp9、apaf-1、SOD、cat和GPX4基因的表达水平。结果:RNA-seq分析显示,OAT患者精子中NANOS1、HSPA6、ALOXE3基因表达升高,BHLHE41、Hey1、PPM1D基因表达下调。姜黄素(20 μM)能有效地保护健康受试者和OAT患者在冷冻保存过程中的精子参数和活力。OAT患者和健康个体冷冻保存的精子均表现出促凋亡基因表达减少,抗凋亡基因表达增加,SOD和GPX4基因水平升高。结论:NANOS1、HSPA6、ALOXE3、BHLHE41、Hey1和PPM1D基因的表达改变可能与OAT的发生有关。此外,姜黄素在冷冻保存期间对健康个体和OAT患者的精子参数都有保护作用。
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来源期刊
Iranian Journal of Public Health
Iranian Journal of Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-
CiteScore
2.20
自引率
7.10%
发文量
300
审稿时长
3-8 weeks
期刊介绍: Iranian Journal of Public Health has been continuously published since 1971, as the only Journal in all health domains, with wide distribution (including WHO in Geneva and Cairo) in two languages (English and Persian). From 2001 issue, the Journal is published only in English language. During the last 41 years more than 2000 scientific research papers, results of health activities, surveys and services, have been published in this Journal. To meet the increasing demand of respected researchers, as of January 2012, the Journal is published monthly. I wish this will assist to promote the level of global knowledge. The main topics that the Journal would welcome are: Bioethics, Disaster and Health, Entomology, Epidemiology, Health and Environment, Health Economics, Health Services, Immunology, Medical Genetics, Mental Health, Microbiology, Nutrition and Food Safety, Occupational Health, Oral Health. We would be very delighted to receive your Original papers, Review Articles, Short communications, Case reports and Scientific Letters to the Editor on the above men­tioned research areas.
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